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一个核心卫星自组装的SERS口味传感器,用于超灵敏地检测AFB

Danni Sun¹, Tao Liu¹, Yiran Yao¹

¹School of Grain Science and Technology, Jiangsu University of Science and Technology, Zhenjiang, 212003, Jiangsu, China.

Mikrochimica acta

|February 26, 2025

概括

开发了一种新的表面增强拉曼散射 (SERS) 适应传感器,用于高度敏感的阿弗拉托克辛B1 (AFB1) 检测. 这种SERS适应传感器使用金纳米星和磁纳米粒子来准确量化食品样本中的AFB1.

科学领域:

分析化学分析化学
纳米技术纳米技术
生物感应是一种生物感应.

背景情况:

非洲毒素B1 (AFB1) 是一种在食品中发现的有毒污染物,对健康构成重大风险.
准确而敏感的AFB1检测方法对于食品安全至关重要.
现有的检测方法可能缺乏灵敏度,特异性,或需要复杂的程序.

研究的目的:

开发一种高度敏感和特定的表面增强拉曼散射 (SERS) 适应传感器用于AFB1检测.
使用金纳米星 (Au NSs) 和Fe3O4@Au纳米粒子 (NPs) 来增强SERS信号和磁分离.
建立一种可靠的方法,在各种食品矩阵中量化AFB1.

主要方法:

用4-aminothiophenol (PATP) 和cDNA作为捕获探头修改的Au NSs的制造.
用AFB1阿普特 (AFB1 Apt) 修改的Fe3O4@AuNP作为信号探针的制备.
核心卫星纳米结构的组装和通过SERS检测磁分离后的信号变化.

主要成果:

在纳米结构组装后,SERS适应传感器显示了显著的信号增强.
检测AFB1涉及到特定的绑定到aptamer,导致纳米结构解离和SERS强度的下降.
对于AFB1的超低检测极限 (LOD) 达到了0.24 pg/mL.
该测试显示,在和阳性食品样本 (大米,玉米,小麦) 的回收和准确性方面表现出色.

结论:

开发的SERS口感传感器为AFB1检测提供了一种高度敏感和特定的方法.
Au NSs,磁性NP和aptamers的组合提供了出色的传感能力和易于分离.
这种方法对食品中AFB1污染的常规监测具有前景,以确保消费者安全.

关键词:

AFB1 是一个空军基地.亚普特美尔 (Aptamer) 是一种药物.澳大利亚NSSs 在Fe3O4@Au NPs中使用Fe3O4@Au 磁分离器的磁性分离方式这就是 SERS SERS.

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