在原始机械分离的家禽肉和从受污染的肉制成的巴氏消毒产品中的Clostridioides difficile
Majda Biasizzo1, Urška Henigman1, Jana Avberšek2
1University of Ljubljana, Veterinary Faculty, National Veterinary Institute, Institute of Food Safety, Feed and Environment, Gerbičeva 60, 1000, Ljubljana, Slovenia.
Anaerobe
|February 26, 2025
概括
在机械分离的肉类中发现了Clostridioides difficile (C. difficile),甚至在一个产品中幸存下来了巴氏杀菌. 这凸显了加工肉制品中的潜在风险.
科学领域:
- 食品安全和微生物学
- 食物传播的细菌病原体.
- 肉制品分析 肉制品分析
背景情况:
- 困难菌 (C. difficile) 是一种重要的食物传播病原体.
- 它经常在各种食品中检测到.
- 机械分离肉 (MSM) 是加工食品中常见的成分.
研究的目的:
- 为了确定C. difficile在和火MSM中的流行率.
- 调查C. difficile在受污染的MSM.产生的巴氏消毒产品中的发生情况.
- 为了描述这些产品中发现的C. difficile分离物.
主要方法:
- 分析了56个MSM样本 (和火) 和6个巴氏消毒肉制品.
- 使用实时PCR和选择性培养进行检测.
- 通过PCR-ribotyping,毒素型和全基因组测序 (WGS) 进行分离物的表征.
主要成果:
- 在16.1%的MSM样本中检测到C. difficile,隔离率为10.7%.
- 一个巴氏化产品的检测结果为C. difficile阳性.
- 六个隔离物显示出不同的PCR-ribotypes,其中五个是毒性;在巴氏杀菌产品中的菌株与原肉来源不同.
结论:
- 困难菌可以存在于原始MSM中,并存活在巴氏消毒过程中.
- 这表明加工肉制品中存在潜在的污染风险.
- 需要进一步的研究来了解C. difficile在肉类加工中的生存和控制.
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