多角头结合西普罗夫洛克萨可以通过抑制NF-κB/IL-6/JAK2/STAT3通路来改善慢性细菌性前列腺炎
Shanshan Fan1, Xurui Zuo1, Xinyue Liu2
1Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, No. 69 Zengchan Road, Hebei District, Tianjin, 300250, China.
Journal of ethnopharmacology
|February 26, 2025
概括
多角头 (Pc) 与西普罗素 (CIP) 结合,通过向Pik3cb并抑制关键炎症通路,有效治疗慢性细菌性前列腺炎 (CBP). 来自Pc的酸 (GA) 被确定为这种协同效应的关键组成部分.
科学领域:
- 药理学和传统医学的医学.
- 泌尿病学和传染病的研究
- 分子生物学和蛋白质组学
背景情况:
- 传统的苗族草药Polygonum capitatum (Pc) 对泌尿疾病,特别是慢性细菌性前列腺炎 (CBP) 的治疗有前途.
- 与PC和Ciprofloxacin (CIP) 联合治疗显示出比CBP单一治疗更高的疗效,但机制尚不清楚.
研究的目的:
- 阐明PC和CIP在治疗CBP中的协同作用机制.
- 确定Pc的主要活性成分,负责CIP的增强治疗效果.
主要方法:
- 建立了CBP的老鼠模型,并用Pc和CIP组合治疗.
- 通过前列腺系数,细菌负载和组织病理学评估治疗效果.
- 使用了西方抹杀,qRT-PCR,RNA-seq,英才途径分析 (IPA),组织热蛋白质组概况 (组织-TPP) 和分子对接.
主要成果:
- Pc-CIP组合显著降低了前列腺炎症,细菌负载,并恢复了组织结构.
- 转录组分析显示NF-κB/IL-6/JAK2/STAT3通路的抑制.
- 组织TPP将Pik3cb确定为直接目标,分子对接证实Pc与Pik3cb结合的酸 (GA) 结合了Pik3cb,从而提高了CIP的疗效.
结论:
- 通过准Pik3cb和抑制NF-κB/IL-6/JAK2/STAT3信号通路,PC和CIP协同减轻CBP的作用.
- 酸 (GA) 被确定为这种组合疗法中Pc的潜在关键药理学成分.
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