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Selwyn Arlington Headley¹, Gustavo Rodrigues Queiroz², Joana Cristina Smaha de Jesus Lima³

¹Laboratory of Animal Pathology, Department of Preventive Veterinary Medicine, Universidade Estadual de Londrina, Londrina, Paraná, Brazil; Multi-User Animal Health Laboratory (LAMSA), Department of Preventive Veterinary Medicine, Universidade Estadual de Londrina, Londrina, Paraná, Brazil.

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概括

这项研究提供了第一次证据,证明绵羊马疹病毒2 (OvGHV2) 感染了绵羊的胎盘,证实了它在胎儿感染和生殖问题中的作用. 这些发现突显了15A免疫组织化学试验在检测这些澳门病毒感染方面的有效性.

科学领域:

兽医病毒学兽医病毒学
动物病理学动物病理学
传染性疾病传染性疾病

背景情况:

Ovine gammaherpesvirus 2 (OvGHV2) 是一种澳门病毒,负责在反动物中引起恶性白内障热 (MCF).
MCF病毒 (MCFV) 综合体包括共享15A抗原表位的澳门病毒.
移植性OvGHV2感染在反动物中很少被记录,在绵羊中没有被记录.

研究的目的:

提供OvGHV2移植感染在绵羊中的证据.
为了调查病毒在生殖感染中的潜在作用.
验证15A免疫组织化学 (IHC) 试验用于检测胎儿组织中的MCFV.

主要方法:

对一名怀孕的圣伊内斯羊和她的两个胎儿进行了尸检.
分析母亲和胎儿器官,使用基于15A的IHC测定MCFV抗原.
聚合酶连锁反应 (PCR) 来放大OvGHV2体蛋白质基因.

主要成果:

病理发现包括羊的脑和肝细胞退化,以及两个胎儿的脑.
通过15A-IHC在母羊和胎儿#2的各种器官中检测到MCFV抗原.
从母亲器官,胎儿1号大脑和胎儿2号肝脏中放大了OvGHV2DNA,证实了两个胎儿的OvGHV2感染.

结论:

这项研究报告了 OvGHV2 相关的第一个在绵羊中被记录的胎盘感染病例.
此外,还证实了澳门病毒在反动物生殖感染中的作用.
15A IHC测定对于检测胎儿器官中的MCFV抗原是有效的.

关键词:

15A抗原表位是15A的抗原表位.澳门病毒澳门病毒澳门病毒恶性白内障发烧恶性白内障发烧怀孕中毒症怀孕中毒症转胎盘感染的感染.

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