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Simultaneous Quantification of T-Cell Receptor Excision Circles TRECs and K-Deleting Recombination Excision Circles KRECs by Real-time PCR
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双模块比作为基于患者的实时质量控制指数,用于小班检测和比较试验

Yuanyuan Li1, Xiaoling Chen1, Ying Zhao1

  • 1Department of Laboratory Medicine, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China; Zhejiang Key Laboratory of Clinical In Vitro Diagnostic Techniques, Hangzhou, China; Institute of Laboratory Medicine, Zhejiang University, Hangzhou, China.

Clinica chimica acta; international journal of clinical chemistry
|February 26, 2025
PubMed
概括

双模块比率通过减少数据变化和检测系统错误来改善基于患者的实时质量控制 (PBRTQC). 这种新的指数可确保实时在多个实验室仪器中得到一致的结果.

关键词:
比较性检测试验 进行比较性检测试验.指数加权移动平均线指数加权移动平均线内部质量控制 内部质量控制基于患者的实时质量控制.质量管理质量管理.

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...