相关实验视频
Updated: May 25, 2025

13:47
Chromatin Immunoprecipitation ChIP using Drosophila tissue
Published on: March 23, 2012
25.1K
检测在不同发育阶段的小鼠丸中素乳酸的分布模式
Qian-Ni Li1, Fei-Chen Wang2, Zhen He2
1College of Life Science, Qinghai Normal University, Xining, Qinghai 810099, China.
Gene
|February 26, 2025
概括
基因表观遗传修饰的关键因素 - - 基因激素乳化 - - 在小鼠的发育过程中存在于小鼠丸中. 它的分布受乳酸脱酶A的影响,影响精子生成和生殖细胞发育.
科学领域:
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 分子生物学分子生物学
- 生殖生物学 生殖生物学
背景情况:
- 乳酸盐是一个关键的代谢物,参与能源生产和信号传输.
- 乳化是一种后翻译性修饰,在表观遗传上影响基因表达.
- 激素乳化在丸发育中的作用在很大程度上是未被探索的.
研究的目的:
- 在小鼠的出生后发育过程中研究乳酸相关酶 (HAT,HDAC,HK2) 的表达.
- 确定在正在发育的丸中各种组织激素乳酸化标记的细胞分布.
- 评估乳酸脱酶A (LDHA) 对基因素乳酸和精子生成的影响.
主要方法:
- 在不同产后天的小鼠丸中分析HATs,HDACs和HK2的基因表达.
- 在丸细胞中免疫组合化学检查组合素乳酸化标记 (H3K9la,H3K12la,H4K18la,H3K5la,H3K14la).
- 对照试验中的基因组乳酸化模式与塞尔托利细胞特异性LDHA淘汰 (Ldha-cKO) 试验的比较.
主要成果:
- 乳化相关的转录表达在发育过程中受到调节.
- 在产后的精子生成细胞和塞尔托利细胞中发现了基因组乳酸化标记H3K9la,H3K12la和H4K18la.
- 在特定的生殖细胞群或成熟的塞尔托利细胞中没有检测到H3K5la和H3K14la.
- 塞尔托利细胞中LDHA的缺失改变了精子生成细胞中素乳酸定位,特别是影响了H4K12la的分布.
结论:
- 在丸发育过程中,基斯乳酸化被动态调节.
- 通过LDHA,塞尔托利细胞衍生的乳酸在调节精子生成至关重要的基因组母乳化模式方面发挥着重要作用.
- 这项研究提供了关于乳化在男性生殖细胞发育和功能中的作用的基础见解.
相关概念视频
Inheritance of Chromatin Structures
6.2K
Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying...
6.2K
Histone Modification
13.0K
The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...
13.0K
Heterochromatin
9.6K
The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at...
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at...
9.6K
Spreading of Chromatin Modifications
8.2K
The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...
Writers
The writer...
8.2K

