增强前体供应和工程流出系统,以改善Yarrowia lipolytica中的酸生产和分泌
Mei-Li Sun1, Yun Xu1, Lu Lin1
1State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, People's Republic of China.
Journal of agricultural and food chemistry
|February 26, 2025
概括
研究人员通过增强前体供应和过度表达载体,优化了酵母中的酸 (ABA) 生产. 这种工程菌株在生物反应器中实现了创纪录的2056.64毫克/升的ABA标位.
科学领域:
- 生物技术是生物技术.
- 合成生物学 合成生物学
- 代谢工程是代谢工程.
背景情况:
- 酸 (ABA) 是一种具有广泛应用的关键植物激素.
- 目前通过真菌发酵生产的ABA在菌株优化和工艺控制方面面临着挑战.
- 开发高效的微生物细胞工厂用于ABA合成是非常可取的.
研究的目的:
- 为了改造Yarrowia lipolytica酵母,以增强新的酸生产.
- 通过代谢工程和过程优化来改善ABA标位.
- 建立一个强大的基于酵母的系统,用于高产量的ABA合成.
主要方法:
- 亚罗维亚脂质菌的代谢工程使用"推-拉-制"策略,准乙-CoA.
- 在分析和过度表达ABC载体 (Yl/Gcn20).
- 优化发酵条件,包括温度和基质添加 (多德干).
主要成果:
- 通过增强前体供应,工程菌株SM309的ABA产量从266.34增加到328.51毫克/升.
- 过度表达YlGcn20进一步提高了ABA产量10.88%,达到354.21 mg/L.
- 综合策略实现了605.92 mg/L的最终标位,并在5L生物反应器中创下了2056.64 mg/L的纪录.
结论:
- 改造的Yarrowia lipolytica菌株代表了基于酵母的ABA生产的重大进步.
- 这项研究证明了综合代谢工程和流程优化的高位植物激素合成的有效性.
- 这项工作为微生物系统中的新ABA生产建立了新的基准.
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