由域混合控制所赋予的可逆性无处不在,在植物免疫中配对NLR免疫受体复合体恒温
Zhiyi Chen1, Jianhua Huang2, Jianyu Li1
1State Key Laboratory of Agricultural and Forestry Biosecurity, Department of Plant Pathology, China Agricultural University, Beijing, China.
Nature communications
|February 26, 2025
概括
植物免疫受体需要严格调节. 一个新的ubiquitination-deubiquitination循环控制RRS1/RPS4复合体的稳态,影响植物的抗病能力和自身免疫力.
科学领域:
- 植物免疫力 植物免疫力
- 分子植物病原体相互作用
- 蛋白质稳态是蛋白质的稳态.
背景情况:
- 植物细胞内核酸结合氨酸丰富的重复 (NLR) 免疫受体对于检测病原体和激活防御至关重要.
- 虽然单独的NLR调节是可以理解的,但对配对NLR复合体恒温的机制仍然不太清楚.
- 阿拉比多opsis RRS1/RPS4 NLR复合体通过 RRS1 的集成 WRKY 域提供抗病能力.
研究的目的:
- 阐明控制配对RRS1/RPS4免疫受体复合物的稳态的调节机制.
- 调查翻译后修改对配对NLR复合体稳定性和功能中的作用.
主要方法:
- 近距离标记技术用于识别相互作用的蛋白质.
- 生物化学试验用于研究无处化和无处化过程.
- 遗传学分析来追踪调节机制的进化历史.
主要成果:
- 一个涉及E3酶RARE和deubiquitinating酶UBP12/UBP13的ubiquitination-deubiquitination循环调节了RRS1/RPS4复杂的稳态.
- 稀有无处不在RRS1的wrky域,促进其降解和破坏RS1/RPS4复合物的稳定.
- UBP12/UBP13通过对RRS1的WRKY域进行二氧化清除来抵消RARE的作用.
- 稀有和UBP12/UBP13也调节了WRKY70和WRKY41转录因子的丰度.
结论:
- 一个新的ubiquitination-deubiquitination循环控制了配对的RRS1/RPS4 NLR复合物的稳态.
- 在NLR中域集成可以重新利用现有的翻译后修改路径来实现新的监管功能.
- 这些发现为维持植物免疫受体功能提供了洞察力,而不会诱导自身免疫.
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