洞察细菌和菌的多样性,宿主范围和时间稳定性
Nejc Stopnisek1,2, Stina Hedžet3, Tomaž Accetto4
1Department for Microbiological Research, Centre for Medical Microbiology, National Laboratory of Health, Environment and Food, Prvomajska 1, Maribor, 2000, Slovenia. nejc.stopnisek@nlzoh.si.
BMC microbiology
|February 26, 2025
概括
这项研究选了肠道细菌Bacteroides和Phocaeicola中的prophages,发现了22种独特的病毒类型. 这些菌体表现出广泛的宿主范围和稳定性,携带抗生素耐药性和毒素的基因.
科学领域:
- 微生物学 微生物学
- 病毒学 病毒学
- 肠道微生物组研究研究
背景情况:
- 菌体 (菌体) 对肠道微生物组动态至关重要.
- 前体,集成的菌体形式,影响细菌进化.
- 在 Bacteroides 和 Phocaeicola 中的食原体多样性不太清楚.
研究的目的:
- 选和表征肠道中的prophages Bacteroides 和 Phocaeicola 属.
- 为了研究prophage多样性,宿主范围和时间稳定性.
- 了解人类肠道中菌体的功能影响.
主要方法:
- 使用了三种生物信息工具:Cenote-Taker 3,Vibrant 和 PHASTER.
- 在孤立的细菌基因组和肠道病毒组数据中分析了菌体.
- 聚类高质量的菌体以识别独特的病毒操作分类学单位 (vOTU).
主要成果:
- 在Bacteroides和Phocaeicola中确定了22个独特的prophage vOTU.
- 前体体表现出比以前观察到的更广泛的宿主范围.
- 在个人和时间点之间检测到某些prophages的时间稳定性.
- 发现属于Caudoviricetes类的prophages含有抗生素耐药性和毒素基因.
结论:
- 多种检测工具可以提高预兆细胞多样性的评估.
- 已识别的prophages是普遍的,稳定的,并拥有广泛的宿主范围.
- 携带耐药性和毒素基因的食原体可能会影响肠道健康和细菌群落.
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