深入研究Colla Corii Asini对皮肤的潜在抗衰老作用:一项比较研究
Fan Wu1,2, Xiao Xie3,4, Wenwen Ru3
1Department of Cosmetics, School of Light Industry and Engineering, Beijing Technology and Business University, Beijing, China.
Journal of cosmetic dermatology
|February 27, 2025
概括
局部Colla Corii Asini (CCA) 改善了皮肤屏障并减少了纹. 与压碎的CCA (CCAC) 相比,CCA寡 (CCAO) 显示出更好的抗衰老效果,这是由于皮肤吸收效果更好.
科学领域:
- 皮肤病学和化品
- 衰老的生物化学 衰老的生物化学
- 皮肤屏障研究研究
背景情况:
- 皮肤衰老的特点是屏障功能障碍和纹形成.
- 口服Colla Corii Asini (CCA) 显示了抗衰老的特性.
- 局部CCA对皮肤衰老的疗效仍未得到充分研究.
研究的目的:
- 为了研究和比较局部CCA粉碎 (CCAC) 和其酶化消化剂,CCA寡 (CCAO) 的抗衰老作用.
- 评估CCAC和CCAO对皮肤屏障功能和纹形成的影响.
- 评估它们对皮肤细胞的影响背后的分子机制.
主要方法:
- CCAC和CCAO分别通过粉碎和酶消化来制备.
- 在体外研究中使用HaCaT和人类皮肤纤维细胞 (HSF) 细胞模型.
- 试验包括MTT检测细胞毒性,抓伤试验检测迁移,qRT-PCR,免疫光,以及ELISA对关键皮肤分子的基因和蛋白质表达分析.
主要成果:
- 在低于2.5mg/ml的度下,CCAC和CCAO没有显著的细胞毒性.
- 这两种治疗都显著增强了HaCaT细胞的增殖和迁移.
- CCAO和CCAC增加了屏障蛋白 (AQP3,FLG,LOR) 和抗蛋白 (COL-1,COL-3,ELN) 的表达,同时降低了MMP-1的调节. CCAO显示出更明显的影响,特别是对FLG,LOR,COL-1,COL-3和ELN.
结论:
- 局部CCA有效地改善皮肤屏障功能,促进原蛋白的产生,增强皮肤弹性,从而减轻皮肤衰老.
- 由于增强的皮肤透和利用,CCA寡 (CCAO) 提供了比CCAC更好的抗衰老效益.
- CCAO代表了一种有前途的局部药物,用于对抗皮肤衰老.
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