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编程CRISPRi来控制菌体T7的生命周期

Tobias Bergmiller1

  • 1Department of Biosciences, Faculty of Health and Life Sciences, University of Exeter, Exeter, United Kingdom.

Frontiers in microbiology
|February 27, 2025
PubMed
概括

集群定期间隔的短Palindromic重复干扰 (CRISPRi) 通过向关键促进体和T7RNA聚合酶,有效控制菌体T7生命周期和传染性. 多重CRISPRi显著增加了溶解时间和减少了斑块大小,证明了其对菌体操纵的潜力.

关键词:
克里斯普里是指克里斯普里.细菌菌体是一种菌体.细菌菌体生命周期dCas9 的使用情况光报道员的光报道员

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