在Desulfurella acetivorans中的propionate代谢
Eugenio Pettinato1, Thomas M Steiner2, Eric A Cassens1
1Institute for Molecular Microbiology and Biotechnology, University of Münster, Münster, Germany.
Frontiers in microbiology
|February 27, 2025
概括
酸硫可以在酸盐上生长,但不使用预测的甲基酸盐循环. 相反, propionate 通过 succinyl-CoA 进入三碳酸 (TCA) 循环,挑战基因组路径预测.
科学领域:
- 微生物学 微生物学
- 代谢工程是代谢工程.
- 生物化学 生物化学
背景情况:
- 酸硫 (Desulfurella acetivorans) 是一种无氧细菌,以减少硫和在乙酸或上生长而闻名.
- 基因组分析表明,D. acetivorans利用甲基酸盐循环进行酸盐代谢.
研究的目的:
- 为了研究Desulfurella acetivorans中的propionate同化代谢途径.
- 在酸盐生长过程中实验验验证预测的甲基酸盐循环活性.
主要方法:
- 在酸上培养D. acetivorans.
- 酶活性测定. 酶活性测定.
- 稳定的同位素标记为 [U-13C3] 酸.
- 比较蛋白质组学分析.
主要成果:
- D. acetivorans在酸盐上生长,但没有检测到甲基酸盐循环酶活性.
- 蛋白质组数据表明,甲基酸盐循环在酸盐生长过程中是不活跃的.
- 乙烯-CoA被碳氧化为甲基乙烯-CoA,然后转化为乙烯-CoA进入TCA循环.
结论:
- 代谢途径的基因组预测需要实验验证.
- 德苏尔福瑞拉乙子使用一种替代的酸盐同化途径,与甲基酸盐循环不同.
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