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L-Carnitine提高了猪精子的质量,寿命,以及冷却精液中的 zona pellucida 结合

Monique de Albuquerque Lagares¹, Nathalia Abreu Amaral², Joyce Junia Braga²

¹Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.

Animal reproduction

|February 27, 2025

概括

将L-carnitine (LC) 添加到野猪精液剂量中,可以显著延长冷藏期间的精子活力. 补充10毫米LC改善了运动性和膜功能,提高了长达12天的可用性.

科学领域:

动物科学动物科学
生殖生物学生殖生物学
生物技术是生物技术.

背景情况:

猪人工授精 (AI) 剂量在17°C保存时通常可存活5天.
延长野猪精液剂量的可行性对于高效的AI程序至关重要.

研究的目的:

调查L-卡尼丁 (LC) 补充剂在长期冷藏期间对野猪精液生存能力的影响.
为了确定最佳的LC度和时间,以改善精子质量和功能.

主要方法:

实验1:评估精子运动性,动力学,膜功能和氧化应激标记物 (过氧化,酸盐) 在精液中,在储存的第五天添加了不同度的LC (0.5-10毫米).
实验2:经过LC补充后评估精子- zona pellucida (ZP) 的结合能力.
实验3:研究了在储存的第1天添加10毫米LC对12天的精子参数的影响.

主要成果:

在第5天添加的10毫米液晶增加了精子运动到第8天,并改善了膜功能.
在第1天添加的10毫米液增加了精子的运动力到第12天,并在第9天降低了亚酸盐水平.
精子-ZP结合在储存的第6天增加了10毫米的LC.

结论:

补充10毫米的L-carnitine有效地提高了野猪精子的生命力和质量,在17°C的液体存储延长期间.
添加LC可以提高精子的运动性,膜完整性和 zona pellucida 的结合,延长可用的精子寿命长达12天.

关键词:

抗氧化剂是一种抗氧化剂.人工授精是一种人工授精.野猪野猪野猪野猪精子精子精子猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪

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