植物病原菌通过保存的酶效应器劫持酸盐信号
Carl L McCombe1, Alex Wegner2, Louisa Wirtz2
1Research School of Biology, The Australian National University, Canberra, ACT, Australia.
概括
致病真菌通过化伊诺西酸盐 (PP-InsP) 来劫持植物酸盐传感器. 这种操纵会触发酸盐饥饿信号,增强植物的真菌疾病症状.
科学领域:
- 分子植物病原体相互作用
- 菌类病原体
- 植物信号通道
背景情况:
- 无机酸盐 (Pi) 对于植物生命至关重要,其可用性通过伊诺西酸盐 (PP-InsP) 水平被感知.
- 植物细胞具有监测酸盐水平的保护机制,这对生长和发育至关重要.
研究的目的:
- 研究致病真菌,特别是Magnaporthe和Colletotrichum物种如何操纵植物酸盐感应.
- 阐明真菌效应器干扰植物Pi恒温的分子机制.
主要方法:
- 来自病原性真菌的保存Nudix酶因子的结构和酶分析.
- 在Magnaporthe oryzae,Colletotrichum higginsianum和Colletotrichum graminicola中进行基因删除实验.
- 对PP-InsP水解和诱导植物酸盐饥饿信号的分析.
主要成果:
- 来自*Magnaporthe*和*Colletotrichum*的一种保存的Nudix酶效应剂家族选择性地化PP-InsP.
- 删除这些Nudix效应器可显著减少多种植物真菌病理系统的疾病症状.
- 这些真菌因子被证明诱导了植物酸盐饥饿信号.
结论:
- 病原性真菌采用一种保存的分子策略来劫持植物酸盐传感途径.
- 菌的Nudix酶因子通过化PP-InsP促进疾病,从而操纵植物的Pi稳态.
- 这种机制代表了多种植物病原菌使用的重要毒性策略.
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