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通过水力动力腔化增强微藻细胞失活:从流细胞计分析的见解

Diyuan Wang¹, Francis L de Los Reyes¹, Joel J Ducoste¹

¹Department of Civil, Construction, and Environmental Engineering, North Carolina State University, 915 Partners Way, Campus Box 7908, Raleigh, NC, 27606, USA.

|February 27, 2025

概括

水力动态化有效地破坏了微藻细胞. 保持细胞残渣低于20%对于有效的无活化至关重要,细胞膜完整性是关键的优化目标.

科学领域:

生物技术是生物技术.
生物工艺工程生物工艺工程
微藻养殖微藻养殖

背景情况:

液动力学化 (HC) 为微藻细胞破坏提供了一个低能耗的替代方案.
高温加热效率因微藻种类和操作条件而异.
量化细胞损伤和优化HC参数对于生物产品采集至关重要.

研究的目的:

为了研究HC对Dunaliella viridis的时间依赖性影响.
在HC下量化细胞破坏和无活化动力学.
确定优化HC治疗的关键生理终点.

主要方法:

对于多次HC通道,使用了长板式化系统.
细胞度,大小分布和计数通过细胞计数和流动细胞计量分析.
使用光探针 (FDA,EB,DCFDA) 评估了细胞活力,代谢活性和活性氧物种 (ROS).

主要成果:

HC有效地破坏和禁用了Dunaliella viridis细胞,遵循伪第一阶动力学.
随着电池碎片的增加,失活率和能源效率下降,需要碎片水平低于10-20%.
细胞膜破裂是失活的主要机制,释放细胞内内容.

结论:

细胞膜完整性是优化微藻生物产品采集中HC协议的关键终点.
一个P-因子模型改善了细胞无活化动力学的预测,而不是仅仅化数.
HC通过破裂膜来使细胞无活化,使可活细胞中的ROS减少.

关键词:

洞穴形成洞穴形成细胞活力细胞活力叶绿素的光效应叶绿素光效应流动细胞计量流动细胞计量微藻类是一种微藻类.反应性氧物种 (ROS) 是一种反应性氧物种.

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相关概念视频

Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...