分析雌性周期动态与不同的协议的4.7毫克deslorelin植入物应用在母狗
Tetiana Holumbiiovska1, Małgorzata Ochota2, Sylwia Prochowska2
1Department of Reproduction with the Clinic of Farm Animals, Faculty of Veterinary Medicine, Wrocław University of Environmental and Life Sciences, Pl. Grunwaldzki 49, 50366, Wroclaw, Poland; Department of Obstetrics, Gynecology and Biotechnology of Animal Reproduction N.a. H. Zverieva, Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnology of Lviv, Pekarska 50, 79010, Lviv, Ukraine.
最佳的德林酸植入物用于犬类雌激诱导需要在晚期麻醉期间 (热后≥160天) 和在LH峰值 (孕激素≥3 ng/ml) 附近去除. 这保证了一个成功的,肥沃的月经周期.
科学领域:
- 兽医医学 兽医医学 兽医医学
- 生殖生物学 生殖生物学
- 内分泌学 在内分泌学.
背景情况:
- 德斯洛林酸植入物越来越多地用于雌性狗的雌性诱导.
- 对于成功诱导雌激素和生育能力而言,罗林植入物注射和切除的最佳时间尚未确立.
研究的目的:
- 为了比较deslorelin酸植入物在早期和晚期anestrus中诱导雌激素的有效性.
- 根据孕激素水平来确定脱离德斯洛雷林植入物的最佳时间,以实现怀孕.
主要方法:
- 使用了七组雌性狗:早期麻醉植入 (G1),晚期麻醉植入,在LH峰值后去除 (G2),晚期麻醉植入,在LH峰值后去除 (G3) 和对照组 (C).
- 用阴道细胞学,激素测量 (孕激素,LH) 和超声波来评估雌激素诱导.
- 用微型VIDASTM装置测量激素水平,使用酶相关光定量 (ELFA).
主要成果:
- 与晚期麻醉 (G2,G3) 相比,在早期麻醉 (G1) 中,雌激素诱导效果较低.
- 只有在晚期麻醉期间植入的组中观察到怀孕,并在LH峰值 (G2) 附近移除.
- 长时间的植入物保留 (G3) 导致荷尔蒙失衡和受孕失败.
结论:
- 德斯洛雷林酸植入物在晚期麻醉期间 (≥160天的热停止后) 施用时,对诱导雌性是最有效的.
- 移除植入物应与LH峰值 (孕激素≥3ng/ml) 相吻合,以确保肥沃的雌激素周期和成功的怀孕.
- 早期的麻醉器植入或延迟移除可以导致荷尔蒙失衡和生育能力下降.
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