在MdMYB1中的内部替代多基解调节了果中的水果颜色
Kaixuan Yu1, Yaxiao Song1, Xiaohu Gao1
1State Key Laboratory for Crop Stress Resistance and High-Efficiency Production/Apple E & T Research Center of Shaanxi Province, College of Horticulture, Northwest A & F University, Yangling 712100, PR China.
Plant science : an international journal of experimental plant biology
|February 27, 2025
概括
果的颜色是由MdMYB1基因调节的. 替代性多基和DNA甲基化控制转录形式,影响安托西亚尼合成和颜色发育.
科学领域:
- 植物分子生物学 植物分子生物学
- 发展水果发展水果发展
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 在果中,MdMYB1基因对于安托西亚宁合成至关重要.
- 异常的MdMYB1转录发生在早期水果变色期间,但机制尚不清楚.
研究的目的:
- 阐明异常MdMYB1转录背后的机制.
- 了解MdMYB1转录变异在果色彩中的作用.
主要方法:
- RNA测序 (RNA-Seq) 和定量实时PCR (qRT-PCR) 用于识别转录形式.
- 3'cDNA末端的快速放大 (3'RACE),以确认转录终结.
- 对可转移元素和多化信号的DNA甲基化分析.
主要成果:
- 确定了两个MdMYB1转录形式,MdMYB1-S (非功能) 和MdMYB1-L (功能).
- 在早期色化中,MdMYB1-S表达高,而MdMYB1-L在晚期色化中占主导地位.
- MdMYB1-S转录通过多基化终结在第2个内核;这个区域和相关的可转移元素在发育过程中显示出甲基化增加.
结论:
- 在MdMYB1中通过DNA甲基化调节的内部替代多基化 (APA),控制从MdMYB1-S切换到MdMYB1-L.
- 这种表观遗传调节机制通过调节酸氨酸合成来促进果的颜色.
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