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一个光生物传感器,带有双重功能DNA探针,准激活的雌激素受体以评估雌激素活性

Qi Zhang¹, Yuewei Li¹, Jiali Li²

¹College of Energy Environment and Safety Engineering, China Jiliang University, Hangzhou, Zhejiang, 310018, China.

Environmental research

|February 27, 2025

概括

一种新的光生物传感器通过模仿雌激素受体 (ER) 相互作用,快速选环境雌激素 (EEs). 这种高通量方法为检测内分泌干扰化学物质的传统测试提供了更快,更强大的替代方案.

科学领域:

环境科学环境科学
生物化学生物化学
分析化学分析化学

背景情况:

内分泌干扰化学物质 (EDC),包括环境雌激素 (EEs),对环境安全和公共健康构成风险.
检测EEs的传统方法通常是昂贵的,耗时的,并且缺乏可重复性.

研究的目的:

开发一种快速,强大的,高通量光生物传感器,用于评估环境化学品的雌激素活性.
模仿雌激素受体 (ER) 信号通路内的分子相互作用,以进行准确的检测.

主要方法:

设计了一个双功能的DNA探针,其中包含了ER结合序列和OliGreen光染料的染料结合序列.
通过OliGreen在与DNA探针复合的激活ER结合时的光异性减小来测量雌激素活性.
生物传感器使用雌激醇,一种ER抗剂和五种新出现的污染物 (RDP,TPHP,TCIPP,OBS,PFOA) 进行了验证.

主要成果:

生物传感器显示了雌醇的剂量反应,EC50为3nM,下一个检测极限为0.5nM.
没有观察到对已知ER抗剂的反应,这表明特异性.
检测新出现的污染物显示结果与细胞测定一致,在的河水样本中恢复率高 (RDP: 103%,TPHP: 93%,OBS: 98%).

结论:

与传统的细胞检测相比,开发的光生物传感器为选雌激素化学物质提供了一种显著更快,更强大,更易于使用的方法.
这种技术为检测环境雌激素和污染物提供了一种有希望的新型高通量方法.

关键词:

生物传感器是一种生物传感器.探测DNA的探测器是DNA的探测器环境中的雌激素.雌激素受体的受体是什么光异质性是一种光异质性.

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