在Citrobacter freundii JH@Pd中促进了硫甲醇细胞外生物降解,通过启动AcrB排泄启动AcrB排泄
Jian Gao1, Jingyu Wu1, Yuancai Chen1
1The Key Lab of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education, School of Environment and Energy, South China University of Technology, Guangzhou Higher Education Mega Centre, Guangzhou 510006, PR China.
Bioresource technology
|February 27, 2025
概括
生物纳米颗粒通过激活排泄来增强Citrobacter freundii中的抗生素降解. 这一过程促进了呼吸和ATP的产生,为处理抗生素废水提供了新的策略.
科学领域:
- 环境微生物学环境微生物学
- 纳米技术的应用.
- 生物修复是一种生物修复.
背景情况:
- 抗生素耐药性是一个日益严重的全球健康问题.
- 污水处理需要有效的抗生素去除方法.
- 使用纳米颗粒的生物方法显示出对环境修复的希望.
研究的目的:
- 研究生物纳米颗粒增强抗生素生物降解的机制.
- 探索质子驱动力 (PMF) 介导的抗生素排泄 (AEP) 激活的作用.
- 为了确定关键的基因和代谢途径参与硫甲醇 (SMX) 降解.
主要方法:
- 进行了批量实验,以测量SMX降解率.
- 使用定量实时PCR (RT-qPCR) 来分析基因表达.
- 途径分析被用来阐明代谢途径.
- 监测了质子动力 (PMF) 的水平.
主要成果:
- 在最佳的PMF (pH6) 时,生物纳米颗粒显著增加了SMX降解率 (81.7μg L-1 mg-1 蛋白d-1).
- 纳米颗粒调节了转录调节器ramA (2.7-3.1倍),激活了AcrB的排泄.
- 对hype和atpD基因的升级表明增强呼吸和ATP合成.
- 代谢活动和细胞外电子输出对SMX生物降解至关重要.
结论:
- 生物纳米颗粒激活了Citrobacter freundii中的PMF介导的排泄,以加强抗生素排毒.
- 该机制涉及对特定基因 (ramA,hype,atpD) 的上调和能量代谢的优化.
- 这项研究提出了一种改善废水中抗生素生物降解效率的新策略.
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