揭示了Crypthecodinium cohnii中通过双重功能△4-脂肪酸脱酸酶的新型依赖氧气的多可萨赫萨酸生物合成途径
Yi-Tong Yao1, Xiao Zhang1, Chen-Yu Wang1
1College of Life Science and Technology, Jinan University, Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Guangzhou 510632, China.
Bioresource technology
|February 27, 2025
概括
这项研究揭示了Crypthecodinium cohnii如何使用一种新的依赖氧气的途径产生多可萨赫萨酸 (DHA). 间歇性氧气限制策略和双功能酶是提高DHA产量的关键.
科学领域:
- 海洋生物学 海洋生物学
- 生物技术是生物技术.
- 生物化学 生物化学
背景情况:
- 克里普特科迪尼 (Crypthecodinium cohnii) 是多可萨赫萨酸 (DHA) 的重要来源,这是一种必需的欧米茄-3脂肪酸.
- 在C. cohnii中,用于将多科萨氨酸 (DPA) 转化为DHA的精确生化途径在很大程度上仍未被阐明.
- 优化DHA生产对于营养和工业应用至关重要.
研究的目的:
- 阐明C. cohnii.中的DPA转化为DHA的机制.
- 为了研究氧气水平对DHA生物合成的影响.
- 为了确定遗传因素,并优化条件,提高DHA的生产.
主要方法:
- 转录组分析是在过度氧气限制 (EOL) 下进行的.
- 分析了基因表达模式,以确定参与DHA合成的关键酶.
- 间歇性氧气限制 (IOL) 被用作一种种植策略.
- 鉴定到的脱酶的酶活性和基质特异性得到了特征.
主要成果:
- 发现了一种新的依赖氧气的DHA生物合成途径.
- 确定了五个△4-脂肪酸脱酶 (FAD4) 基因.
- 基因CcFAD4_52534表现出双功能活性,将乙酸 (EPA) 转化为DPA,然后转化为DHA.
- 与标准条件相比,IOL策略显著提高了DHA产量.
结论:
- 双功能CcFAD4_52534酶在DPA-DHA转化途径中发挥着至关重要的作用.
- 氧气的可用性是DHA生物合成的关键调节因素.
- IOL策略和C. cohnii的基因工程具有可持续,高产量的DHA生产的巨大潜力.
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