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从Scylla spp.中分离的Enterococcus avium MC09病原性的表型和基因组分析. (泥) 在泰国市场

Sirikan Suwannasin¹, Kamonnut Singkhamanan², Rattanaruji Pomwised³

¹Department of Biomedical Sciences and Biomedical Engineering, Faculty of Medicine, Prince of Songkla University, Hat Yai, Songkhla, 90110, Thailand; Division of Biological Science, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, Thailand.

Microbial pathogenesis

|February 27, 2025

概括

这项研究确定了Enterococcus avium MC09的抗生素耐药性和病原性特征,这种细菌在泰国泥中发现. 海鲜可能充当这种耐药细菌的储存库,构成公共卫生风险.

科学领域:

微生物学微生物学
基因组学就是基因组学.
食品安全食品安全

背景情况:

虫虫 (Enterococcus avium) 是一种已知具有抗生素耐药性的阳性细菌.
海鲜可能是抗生素耐药细菌的储存库,这引起了公众健康的关注.
Enterococcus avium MC09从泰国市场的一只泥中分离出来.

研究的目的:

分析Enterococcus avium MC09的抗生素耐药性和病原性潜力.
调查E. avium MC09中抗生素耐药性和毒性的遗传基础.
评估与海鲜作为抗生素耐药细菌来源相关的风险.

主要方法:

使用Kirby-Bauer盘扩散进行抗生素敏感性测试.
使用牛津纳米孔技术 (ONT) 进行基因组DNA测序.
识别耐药性和毒性相关基因,以及插入序列.

主要成果:

E. avium MC09表现出对克林达米辛,红红素,链杆菌素和四环素的耐药性.
该菌株显示阿尔法血解,表明潜在的毒性.
基因组分析揭示了耐药基因 (ermB,tetL,tetM) 和许多与病毒性相关的基因和插入序列.

结论:

Enterococcus avium MC09具有显著的抗微生物耐药性和致病潜力.
海鲜环境可以容纳抗生素耐药的细菌,如E. avium.
监测海鲜相关细菌的抗生素耐药性对于公共卫生至关重要.

关键词:

抗生素耐药性抗生素耐药性生物信息学分析它们是Enterococcus avium.病毒性因素是病毒性因素.

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