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一种稳定可靠的视觉技术,用于现场检测尼帕病毒核酸

Wencong Chen¹, Li Cai², Danni Ye¹

¹Wenzhou Key Laboratory for Virology and Immunology, Institute of Virology, Wenzhou University, Chashan University Town, Wenzhou, 325000, China.

Scientific reports

|February 27, 2025

概括

一个新的护理点核酸检测 (POC-NAD) 系统在现场提供了快速而准确的尼帕病毒 (NiV) 诊断. 这种创新工具集成RT-PCR,侧流免疫检测和微流体检测,用于可靠的NiV监测.

科学领域:

病毒学病毒学
分子诊断学分子诊断
生物技术是生物技术.

背景情况:

尼帕病毒 (NiV) 是一种高度致命的亚洲特有动物性传染病原体.
随着人类传播的增加,需要对NiV进行快速,现场部署的诊断方法.
当前的诊断方法可能缺乏有效的实地监测所需的速度和可访问性.

研究的目的:

开发和验证尼帕病毒 (NiV) 的护理点核酸检测 (POC-NAD) 系统.
为了能够快速,准确和可靠地对NiV感染进行现场诊断.
促进在资源有限的环境中及时采取NiV监测和控制措施.

主要方法:

设计针对 NiV. 的保存的 G 和 P 基因的特定原料.
将单步RT-PCR,横流免疫试验和微流体技术集成到POC-NAD系统中.
使用模拟临床样本评估系统的性能,并评估其检测极限 (LoD).

主要成果:

开发的POC-NAD系统表现出高的特异性和灵敏度,LOD为199.1个副本/rxn.
初始剂可以同时检测NiV-M和NiV-B菌株.
在21个模拟临床样本中观察到与RT-PCR结果100%的一致性,通过横向流动可视化改善了显示时间和可读性.

结论:

POC-NAD系统是一个适合实时核酸测试尼帕病毒 (NiV) 的工具.
微流体集成确保在各种现场条件下保持一致的性能.
这种诊断工具在资源有限的环境中对NiV监测非常有价值.

关键词:

双目标检测双目标检测侧流免疫染色学侧流免疫染色学尼帕病毒是尼帕病毒.在现场进行现场检查.一步RT-PCR是一个步骤.

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