相关实验视频

Updated: May 25, 2025

Author Spotlight: Optimizing Digital Droplet PCR Method for Accurate Adeno-Associated Viral Genome Quantification
04:43

Author Spotlight: Optimizing Digital Droplet PCR Method for Accurate Adeno-Associated Viral Genome Quantification

Published on: October 11, 2024

1.7K

通过RT-PCR量化犀牛病毒基因组和负链RNA

Shahina Wiehler1, Michelle E Love1, David Proud2

  • 1Department of Physiology and Pharmacology, and Snyder Institute for Chronic Diseases, Cumming School of Medicine, University of Calgary, Calgary, AB, Canada.

Methods in molecular biology (Clifton, N.J.)
|February 27, 2025
PubMed
概括

开发了用于犀牛病毒RNA的定量RT-PCR测定. 这些方法测量了基因组和负链RNA,有助于理解病毒复制和免疫反应激活.

关键词:
气道上皮质 气道上皮质人类犀利病毒.有负链RNA的RNA.实时PCR基因组RNA实时PCR基因组RNA

更多相关视频

Detection and Genogrouping of Noroviruses from Children's Stools By Taqman One-step RT-PCR15:16

Detection and Genogrouping of Noroviruses from Children's Stools By Taqman One-step RT-PCR

Published on: July 22, 2012

17.2K
Development and Validation of a Quantitative PCR Method for Equid Herpesvirus-2 Diagnostics in Respiratory Fluids09:57

Development and Validation of a Quantitative PCR Method for Equid Herpesvirus-2 Diagnostics in Respiratory Fluids

Published on: March 17, 2016

28.2K

相关概念视频

Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
56.7K