通过合成还原型甘氨酸途径固定单碳,超过了卡尔文循环的产量
Beau Dronsella1,2, Enrico Orsi3, Helena Schulz-Mirbach4
1Systems and Synthetic Metabolism, Max Planck Institute of Molecular Plant Physiology, Potsdam, Germany. beau.dronsella@mpi-marburg.mpg.de.
Nature microbiology
|February 27, 2025
概括
利用减少性甘氨酸途径的工程微生物在酸盐和CO2上获得了更高的生物质产量. 这种合成代谢方法为生物生产的食品,燃料和化学品提供了更可持续的途径.
科学领域:
- 合成生物学 合成生物学
- 微生物的新陈代谢
- 生物技术是生物技术.
背景情况:
- 甲酸盐和二氧化碳等一碳化合物是潜在的可再生原料.
- 在Cupriavidus necator中,本地Calvin-Benson-Bassham循环对于格式利用是能量低效的.
- 可持续的微生物生产需要有效的碳固定途径.
研究的目的:
- 为了设计Cupriavidus necator,以增强格式和二氧化碳的增长.
- 取代本地卡尔文-本森-巴什姆循环,使用减小型甘氨酸路径.
- 提高生物质产量,以实现可持续的生物生产.
主要方法:
- 在Cupriavidus necator的基因工程.
- 将卡尔文-本森-巴什姆循环替换为减少性甘氨酸路径.
- 在化学静态中培养,使用甲酸盐和二氧化碳作为唯一的碳来源.
主要成果:
- 改造后的菌株在酸盐和二氧化碳上显示出增长.
- 与野生类型相比,实现了17%更高的生物质产量.
- 在产量方面,在使用卡尔文周期的自然格式养物中表现优于自然格式养物.
结论:
- 降解性甘氨酸途径提高了在一碳化合物上微生物生长的能量效率.
- 合成代谢为可持续的生物生产提供了一个可行的战略.
- 工程Cupriavidus necator显示了使用可再生原料的工业应用的潜力.
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