在氧酸盐结石结石中识别微环境特征时使用多组的自我控制研究
Shang Xu1, Zhi-Long Liu1, Tian-Wei Zhang1
1Department of Urology, The Affiliated Hospital of Qingdao University, Qingdao, Shandong Province, 266000, China.
BMC nephrology
|February 27, 2025
概括
蛋白质组和代谢组分析显示,在患有氧酸盐结石的患者中,尿道的微环境是不同的. 受结石影响的脏显示炎症和氧化应激增加,为结石形成机制提供了洞察力.
科学领域:
- 生物化学 生物化学
- 泌尿器科 泌尿器科 泌尿器科 泌尿器科
- 蛋白质组学是指蛋白质组学.
- 代谢学 代谢学 代谢学
背景情况:
- 氧化酸的结石是一种常见的疾病.
- 尿路微环境在结石形成中起着至关重要的作用.
- 了解这种微环境是开发向治疗的关键.
研究的目的:
- 在患有单边氧酸盐结石的患者中识别尿道微环境的特定变化.
- 阐明与结石形成相关的蛋白质和代谢学概况.
- 为了解结石发育背后的病理生理机制提供见解.
主要方法:
- 双边脏骨盆尿样采集通过囊镜引导的尿道导管.
- 液体染色学-并联质谱学 (LC-MS/MS) 用于蛋白质和代谢分析.
- 生物信息分析包括GO,KEGG,Reactome和PPI网络分析,用于功能解释.
主要成果:
- 36种蛋白质上调,4种蛋白质下调,10种代谢物在受石头影响的尿液中上调.
- 与炎症途径和补充/凝固级联相关的高调蛋白质.
- 上调代谢物主要与氧化应激有关.
结论:
- 蛋白质组和代谢组概况提供了对结石病理生理学的准确反映.
- 已识别的途径为治疗干预提供了潜在的目标.
- 尿路微环境分析对于了解石头的形成和发育至关重要.
相关概念视频
Study Designs in Epidemiology
1.6K
Epidemiological study designs are fundamental tools for investigating the distribution, determinants, and control of health conditions in populations. They help researchers understand the relationships between exposures and outcomes, and they broadly fall into two categories: "observational" and "experimental" studies.
Observational studies are those where the researcher does not intervene but rather observes natural variations. They include cross-sectional, cohort, and...
Observational studies are those where the researcher does not intervene but rather observes natural variations. They include cross-sectional, cohort, and...
1.6K
Urinary Tract Calculi I: Introduction
1.1K
Renal calculi, or kidney stones, are solid deposits of minerals and salts formed inside the kidneys. In medical terminology, "calculus" refers to the stone itself, while "lithiasis" describes the process of stone formation. Depending on their location within the urinary system, these stones may be classified as either urolithiasis, when situated within the urinary tract, or nephrolithiasis, when located within the kidneys. Each term signifies the specific impact of the stone.Predisposition...
1.1K
Urinary Tract Calculi II: Pathophysiology and Clinical Manifestations
860
Renal calculi, commonly termed kidney stones, are crystalline solid masses that form in the kidneys but can occur at any point within the urinary system, encompassing the kidneys, ureters, bladder, and urethra.The pathophysiology of renal stones involves several key factors: supersaturation of the urine with stone-forming constituents, changes in urine pH, a decrease in urine volume, and the presence of substances that promote or inhibit stone formation.Supersaturation of Urine: This is the...
860
Urinary Tract Calculi III: Medical Management
400
The diagnosis of renal calculi involves several imaging techniques, including non-contrast CT scans and ultrasound. These methods help visualize kidney stones, assess their size and location, and detect possible obstructions. Additionally, Measuring urine pH is useful for diagnosing specific stone types, such as struvite (alkaline pH) and uric acid stones (acidic pH). Cystine stones are primarily linked to cystinuria, a genetic condition. A urinalysis helps detect blood in the urine (hematuria)...
400
Urinary Tract Calculi IV: Nutrition Therapy and Prevention
762
Management of renal calculi focuses on effective strategies like tailored nutrition and hydration therapy. Adjusting diet and fluid intake reduces stone formation and recurrence, making these interventions simple yet powerful in kidney stone prevention and management.Understanding Kidney StonesKidney stones form when calcium, oxalate, uric acid, and cystine concentrate and crystallize in urine. Factors contributing to their formation include genetic predisposition, certain medical conditions,...
762
Imaging Studies I: Kidney, Ureter, and Bladder Studies
871
Kidney, Ureter, and Bladder (KUB) StudiesKidney, Ureter, and Bladder (KUB) studies are standard diagnostic imaging procedures used to assess the anatomy of the urinary system. They are commonly utilized for patients experiencing abdominal pain or urinary symptoms. By using a simple X-ray of the abdomen, KUB studies can reveal structural and pathological abnormalities within the kidneys, ureters, and bladder. These studies are particularly valuable in diagnosing kidney stones, urinary...
871


