TIP2-UDT1-OsUPEX1/2模块调节了大米的壁纸发育和功能.
Ruifeng Wang1, Yaqian Sun1, Wanlin Liu1
1Joint International Research Laboratory of Metabolic & Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 20040, China.
米的发育依赖于表皮,由转录因子UDT1和TIP2.2调节. 这些因素形成了一个复杂的,激活下游基因对于花粉生育至关重要.
科学领域:
- 植物生殖生物学 植物生殖生物学
- 分子遗传学 分子遗传学
- 农业科学 农业科学
背景情况:
- 面膜是重要的前壁层,对植物生育和花粉发育至关重要.
- 带状发育是由转录因子和信号通路主导的,UDT1,TIP2,TDR和EAT1在米中形成已知的级联.
研究的目的:
- 阐明大多未知的UDT1和TIP2依赖的监管网络在早期的水其他发展.
- 确定TIP2和UDT1的下游目标,这对于皮膜功能和男性生育能力至关重要.
主要方法:
- 使用淘汰突变物进行功能分析.
- 关键基因的时空表达分析.
- 研究蛋白质与蛋白质相互作用 (异种化).
主要成果:
- TIP2和UDT1在中间层和壁膜中表现出重叠的表达,TIP2对UDT1.1具有表达作用.
- TIP2和UDT1异构,激活下游基因,包括编码银河系转移酶的OsUPEX1和OsUPEX2.
- OsUPEX1和OsUPEX2在功能上是多余的,对皮膜的发育,功能和分泌至关重要.
结论:
- 通过异构化和下游目标的激活,TIP2和UDT1在早期的水发展中发挥着至关重要的作用.
- OsUPEX1和OsUPEX2被确定为TIP2-UDT1通路的关键下游影响者,对面膜功能和大米雄性生育至关重要.
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