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LncPrep + 96kb通过上调prolyl oligopeptidase表达的调节来抑制卵巢纤维化

Hongdan Zhang¹, Jing Wang¹, Jianwei Liu¹

¹School of Basic Medical Sciences, Jiangxi Medical College, Nanchang University, Nanchang, Jiangxi 330019, P.R. China.

Molecular medicine reports

|February 28, 2025

概括

在小鼠中,长非编码RNA LncPrep + 96kb 的损失导致卵巢纤维化和生育能力下降. 基酸酶 (POP) 可能是调解这些纤维化效应的关键标.

科学领域:

生殖生物学生殖生物学
分子内分泌学分子内分泌学
遗传学遗传学是一个

背景情况:

LncPrep + 96kb是一个长的非编码RNA,存在于小鼠粒状细胞中.
一个特定的LncPrep + 96kb片段可以抑制芳酶表达和雌激素分泌.

研究的目的:

调查LncPrep + 96kb在卵巢功能和生育能力中的作用.
探索LncPrep + 96kb对卵巢纤维化影响的分子机制.

主要方法:

产生LncPrep + 96kb敲击 (KO) 的小鼠.
生育能力监测和超排卵测试.
西里乌斯红色染色,西部斑点和RT-qPCR评估卵巢纤维化.
分析普罗利寡酶 (POP) 表达及其下游目标.

主要成果:

LncPrep + 96kb-KO小鼠表现出显著的卵巢纤维化和女性生育能力下降.
通过组织学和分子分析证实了卵巢纤维化.
发现LncPrep + 96kb与POP基因相邻,其淘汰减少了POP表达.
与POP调节的纤维化相关的因素:矩阵金属蛋白酶2 (MMP2),转化生长因子β1 (TGF-β1) 和过氧体增殖器激活受体γ (PPAR-γ).

结论:

在小鼠中,LncPrep + 96kb 缺乏导致卵巢纤维化升高和生育能力受损.
普罗利寡酶 (POP) 是LncPrep + 96kb的一个潜在标.
通过调节POP,LncPrep + 96kb可能会调节卵巢纤维化,这反过来影响PPAR-γ,MMP2和TGF-β1的表达.

关键词:

在 lncPrep + 96kbbb 中.矩阵金属蛋白酶2的基因卵巢纤维化卵巢纤维化过氧体增殖器激活受体 γ 的受体.普罗利尔寡酸酶的使用.转化生长因子β1的转化生长因子β1

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