功能化石墨碳化物作为一个高效的电分析平台,用于在唾液样本中无标签的电化学传感英尔列乌金-8
Sumit K Yadav1,2, Amit K Yadav1, Ajeet Kaushik3
1NanoBio Laboratory, Special Center for Nanoscience, Jawaharlal Nehru University, New Delhi-110067, India. partima@mail.jnu.ac.in.
Nanoscale
|February 28, 2025
概括
这项研究开发了一种使用石墨碳化物 (g-C3N4) 进行早期口腔癌检测的高度敏感的生物传感器. 这种新型平台在真实样本中准确识别了关键的癌症生物标志物 - - 互白素-8 (IL8),这是一种关键的癌症生物标志物.
科学领域:
- 纳米材料科学 科学 纳米材料科学
- 生物医学工程 生物医学工程
- 分析化学 分析化学
背景情况:
- 头癌的高死亡率和转移潜力需要早期检测方法.
- 介乐-8 (IL8) 是一种与口腔癌相关的细胞因子,使其成为诊断生物传感器的目标.
- 石墨碳化物 (g-C3N4) 由于其独特的电子和电化学特性,为电化学生物传感提供了有前途的特性.
研究的目的:
- 开发一种超灵敏的电化学生物传感器,用于检测互白素-8 (IL8).
- 为了利用3-aminopropyl trimethoxysilane (APTES) 功能化的石墨碳化物 (g-C3N4) 作为核心纳米材料.
- 验证生物传感器在实验室和现实世界生物样本中检测IL8的性能.
主要方法:
- 通过尿素的热热解合成g-C3N4.
- 使用APTES对g-C3N4进行功能化,并随后沉积在ITO电极上.
- 使用EDC/NHS化学方法将抗IL8抗体固定在APTES@g-C3N4/ITO电极上.
- 通过牛血清白蛋白 (BSA) 阻止非特异性的结合部位.
- 使用微分脉冲电压测量 (DPV) 进行电化学表征和性能评估.
主要成果:
- 制造的BSA/anti-IL8/APTES@g-C3N4/ITO生物传感器显示了IL8的广泛检测范围 (500 fg mL-1至160 ng mL-1).
- 实现了0.04 ng mL-1的低检测极限 (LOD) 和高灵敏度 (0.015 mA log10 [ng mL-1] cm-2).
- 在分析真实生物样本时,表现出高达10周的良好稳定性和有效的性能.
结论:
- 基于APTES功能化的g-C3N4开发的电化学免疫传感平台显示出对敏感和可靠的IL8检测具有重大潜力.
- 生物传感器的有效性归因于g-C3N4的特性和特定抗体固定化的协同效应.
- 这项技术为口腔癌的早期诊断和临床检测提供了一个有前途的途径.
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