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解码青春期前卵细胞成熟的分子景观:GTPBP4作为体外发育能力的关键驱动因素

Jianpeng Qin¹, Yaozong Wei¹, Ao Ning¹

¹State Key Laboratory of Swine and Poultry Breeding Industry, Key Laboratory of Livestock and Poultry Multiomics, Ministry of Agriculture and Rural Affairs, Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, College of Animal Science and Technology, Sichuan Agricultural University Sichuan, Chengdu, China.

Cell proliferation

|February 28, 2025

概括

了解山羊的卵细胞成熟揭示了像GTPBP4这样的关键基因,这些基因可以改善青春期前卵细胞的发育,为人类生育能力的保护和动物繁殖提供了洞察力.

科学领域:

生殖生物学生殖生物学
分子遗传学分子遗传学
翻译医学是一种翻译医学.

背景情况:

人们对卵细胞成熟机制的了解很少,特别是在家用动物和青春期前女孩的临床应用中.
卵巢组织的冷保存,以保护青春期前女孩的生育能力,需要对发育潜力的科学验证.

研究的目的:

在成年山羊和青春期前山羊的卵细胞成熟期间全面描述转录和转化动态.
为了确定前潮期卵细胞成熟的关键调节因素,并验证它们的功能.
为改善人类生育保护策略和家禽生殖效率提供见解.

主要方法:

成人和青春期前山羊卵细胞的转录组和转录组测序.
基因表达的比较分析.
在体外成熟实验验验证基因功能 (GTPBP4).

主要成果:

确定了基因表达调节从细胞质过程到成熟过程中的染色体分离的顺序转移.
发现了青春期前卵细胞成熟的关键调节基因,包括GTPBP4,TOMM7,CKS2,CCP110,CKAP5,ESCO1,CENPE,CENPF,CENPN和SGO2.
证明GTPBP4通过促进细胞周期进展,器官成熟和mRNA转化,显著提高了青春期前山羊卵细胞发育能力.

结论:

羊卵细胞成熟的详细分子地图,突出成人和青春期前阶段之间的差异.
确定GTPBP4是提高青春期前卵细胞能力的关键因素.
这些发现支持了青春期前女孩的潜在生育保护策略,以及养动物的进步.

关键词:

在GTPBP4中使用.成年山羊的成年山羊是什么意思卵细胞成熟的过程青春期前的山羊青春期前的山羊转录组 (transcriptome) 是一个转录组.翻译ome可以翻译ome.

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