牛多态核中性粒细胞对卡利科福达布尼伊的细胞免疫反应
Liliana M R Silva1,2,3, Sara López-Osorio1,4, Raquel Peixoto1
1Institute of Parasitology, Biomedical Research Center Seltersberg, Justus Liebig University Giessen, Giessen, Germany.
Frontiers in immunology
|February 28, 2025
概括
卡利科福龙达布尼伊抗原弱激活牛中性粒细胞,并诱导有限的中性粒细胞细胞外陷 (NET) 形成. 这表明寄生虫分子可能会干扰神经细胞防御在帕兰菲斯托莫斯感染期间.
科学领域:
- 兽医免疫学 兽医免疫学
- 寄生虫学的寄生虫学
- 细胞免疫学 细胞免疫学
背景情况:
- 欧洲Calicophoron daubneyi感染病例正在增加,对牛群构成重大威胁.
- 中性粒细胞在宿主防御中起着至关重要的作用,包括形成对寄生虫的中性粒细胞外陷 (NETs).
- 了解细胞水平上的宿主-寄生虫相互作用对于控制寄生虫疾病至关重要.
研究的目的:
- 通过Calicophoron daubneyi抗原 (CdAg) 和蛋进行体外牛中性粒细胞激活的研究.
- 确定CdAg诱导牛中性粒细胞中性粒细胞外细胞陷 (NET) 形成的能力.
- 评估CdAg对中性粒细胞功能的影响,包括活性氧物种 (ROS) 生产和腺三酸盐 (ATP) 水平.
主要方法:
- 牛中性粒细胞在体外暴露于CdAg的不同度.
- 用扫描电子显微镜 (SEM),免疫光和细胞外DNA测量来评估中性粒细胞激活和NET形成.
- 通过测量氧气消耗,细胞外酸化,ROS生产和ATP水平来评估中性粒细胞的功能.
主要成果:
- CdAg刺激导致短NETs的弱形成和细胞外DNA的增加,表明早期NETosis.
- 高度的CdAg (100μg/mL) 显著增加了氧气消耗和细胞外酸化,表明剂量依赖的中性粒细胞激活.
- 在暴露于高度CdAg时,牛中性细胞ATP水平显著下降,类似于阳性对照.
结论:
- 卡利科福龙达布尼伊抗原引起牛中性粒细胞的弱激活反应.
- 观察到的弱NET形成表明,C.daubneyi可能具有逃避中性粒细胞介导免疫力的机制.
- 需要进一步的ex vivo研究来确认寄生虫分子是否会在paramphistomosis期间在体内干扰中性粒细胞的功能.
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