使用宽带电阻谱法对活的单个酵母细胞与死亡单个酵母细胞进行无标签的分化
Amirhossein Favakeh1, Amir Mokhtare1, Mohammad Javad Asadi2
1Food Science Department, College of Agriculture and Life Sciences (CALS), Cornell University, Ithaca 14853, New York, USA. alireza@cornell.edu.
Lab on a chip
|February 28, 2025
概括
本研究介绍了一种使用电阻谱学 (EIS) 分析单细胞的新型微流体平台. 该系统通过测量它们在3 GHz的细胞质阻抗来区分活的和死的酵母细胞.
科学领域:
- 生物物理学的生物物理.
- 微流体学 微流体学
- 电气工程 电气工程
背景情况:
- 无标签和非侵入性方法对于监测细胞特性至关重要.
- 电阻谱学 (EIS) 提供了一种有前途的方法来探测细胞和细胞内特征.
- 微流体平台能够精确地操纵和分析单个细胞.
研究的目的:
- 开发和演示一个新的无微流体平台,与共平面波导相集成,用于使用EIS进行单细胞分析.
- 根据其内在的电气特性,研究EIS在区分活细胞和死细胞方面的潜力.
- 建立一种实时监测细胞对外部刺激反应的方法.
主要方法:
- 开发一个微流体芯片,集成共平面波导,用于EIS测量.
- 使用介电泳和水力动力聚焦来精确地捕捉单细胞.
- 在单个 * Schizosaccharomyces pombe * (裂变) 酵母细胞上进行宽带 EIS 测量 (30 kHz 到 6 GHz).
- 分析双端口散射 (S) 参数以确定阻抗特性.
主要成果:
- 成功捕获和电气表征单个酵母细胞.
- 在3GHz的频率下,在活和死酵母细胞之间的细胞质阻抗中显示出明显的区别.
- 验证了平台能够感知微妙的电气性质变化的能力.
结论:
- 开发的基于EIS的微流体平台是有效的无标签,非侵入性单细胞分析.
- 这项技术可以根据阻抗特征区分细胞生存能力.
- 该平台具有实时细胞诊断和监测细胞反应的潜力.
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