鉴定和功能分析新型SNP在Enterocin基因中的Enterococcus faecium GHB2121的新型SNP
Mohamed Merzoug1, Khédidja Mosbahi2, Daniel Walker2
1Higher School of Biological Sciences of Oran, BP 1042 Saim Mohamed, Cité Emir Abdelkader (EX-INESSMO), 31000, Oran, Algeria. merzoug.mohamed1@yahoo.fr.
Probiotics and antimicrobial proteins
|February 28, 2025
概括
分析了Enterococcus faecium GHB21的素基因中的单核酸多态性 (SNP). 尽管发生了突变,但氨酸保留了结构完整性和对食品安全和病原体控制的抗菌疗效.
科学领域:
- 微生物学 微生物学
- 遗传学 是一个遗传学.
- 生物化学 生化学
背景情况:
- 菌 (Enterococcus faecium GHB21) 产生强大的抗微生物,称为菌素.
- 菌素,主要是IIa类细菌素,对Listeria monocytogenes等病原体表现出活性.
- 这些特性使得素在食品防腐和治疗应用中具有价值.
研究的目的:
- 研究单核酸多态 (SNPs) 在E. faecium GHB21.21的素和免疫基因中的功能和结构影响.
- 分析关键的素基因 (entA,entB,entP) 和它们相关的免疫基因 (entAi,entPi) 的特定突变.
主要方法:
- 用克隆和测序来识别遗传变异.
- 生物信息学工具,包括蛋白质变异效应分析仪和DynaMut,用于功能影响评估.
- 分析了结构特征,如二硫化物键和β-sheet安排.
主要成果:
- 确定了两种新型SNP,导致在EntPi中的前素P (EntP) 中的G15N和V36I氨基酸替代.
- 还分析了成熟EntP中的V9I突变和EntAi中的G48S突变.
- 生物信息学分析将突变归类为中性,V9I稳定了EntP,但降低了灵活性;关键的结构特征保持不变.
结论:
- 来自E. faecium GHB21的内素尽管发现了遗传变异,但表现出了显著的结构弹性.
- 这些发现支持素在食品安全和打击多药耐药病原体方面的潜在应用.
关键词:
肠球菌 (Enterococcus faecium) 是一个有毒的菌株.具有抗微生物活性的抗菌活性.克隆,就是克隆人类.氨酸基因是氨酸的基因之一.国家统一计划 (SNP) 是一个国家统一计划.结构预测结构预测更多相关视频
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