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相关概念视频

Meiosis I01:49

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Meiosis is a carefully orchestrated set of cell divisions, the goal of which—in humans—is to produce haploid sperm or eggs, each containing half the number of chromosomes present in somatic cells elsewhere in the body. Meiosis I is the first such division, and involves several key steps, among them: condensation of replicated chromosomes in diploid cells; the pairing of homologous chromosomes and their exchange of information; and finally, the separation of homologous chromosomes by...
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Induction and Validation of Cellular Senescence in Primary Human Cells
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提升的衰老标志物在发展三体瘤 21 人类肺部.

Randa Belgacemi1, Caroline Cherry1, Michael Thompson2

  • 1The Lundquist Institute, Pediatrics, Torrance, California, United States.

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概括

人类三症包括唐氏综合征 (T21) 与胎儿肺部发育中的细胞衰老和DNA损伤有关. 这项研究显示,衰老标志物和氧化应激增加,特别是在T21肺部.

关键词:
唐氏综合征是什么意思肺部发育 肺部发育 肺部发育衰老是一种老化.三重症是三重症的一种.

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科学领域:

  • 遗传学 遗传学是一种遗传学.
  • 发展生物学 发展生物学
  • 细胞生物学 细胞生物学

背景情况:

  • 人类染色体异常,如三症,显著影响基因表达,并导致各种细胞和器官表型.
  • 增加细胞衰老 (SEN) 和氧化应激是在三症状况中出现的新问题.

研究的目的:

  • 为了研究细胞衰老,衰老相关的分泌表型 (SASP) 和氧化应激在人类胎儿肺组织和纤维细胞与三形 13,18 和 21.
  • 在产前肺部发育过程中,建立三症和这些细胞压力标志物之间的关系.

主要方法:

  • 对人类胎儿肺组织和原发性纤维细胞的分析,这些纤维细胞来自三综合症13,18和21.
  • 端粒酶相关焦点 (TAF) 染色用于DNA损伤.
  • 对于SEN和SASP标记基因表达的RT-qPCR (CDKN2B,IL-6,CXCL8,CDKN2A). 这种基因表达的基因表达的基因表达的基因表达的基因表达的基因表达.
  • γ-H2AX染色用于DNA损伤.
  • 使用MitoSOX和CellROX进行反应性氧物种 (ROS) 的评估.

主要成果:

  • 在T21和T18肺部观察到DNA损伤 (TAF,γ-H2AX),在所有三症组中,尤其是T21中,γ-H2AX上调.
  • 三位体21 (T21) 和T18肺部显示SEN标志物CDKN2B和SASP标志物IL-6和CXCL8.8的增加.
  • 三胞胎症13 (T13) 肺部表现出CDKN2A上调,没有显著的SASP变化.
  • 在T21纤维细胞中,SEN标志物,P21表达和ROS水平增加.
  • 来自T13和T18的纤维细胞也显示了增加的γ-H2AX阳性细胞.

结论:

  • 这项研究提供了第一个证据,将细胞衰老和三症异常在产前人类肺部发育过程中联系起来.
  • 三合体21显示出与衰老标志物升高,DNA损伤和氧化应激在发育肺组织中的显著关联.
  • 这些发现突出了潜在的细胞机制,这些机制是肺发育中三症的表型后果的基础.