激活MSB2的费罗蒙通路调节真菌的血膜完整性,以应对除草剂辅助剂的作用
Jianwei Pu1, Xiuju Long1, Yifan Li1
1Jiangsu Provincial Key Lab for Solid Organic Waste Utilization, Key Lab of Organic-based Fertilizers of China, Jiangsu Collaborative Innovation Center for Solid Organic Wastes, Educational Ministry Engineering Center of Resource-saving fertilizers, Jiangsu Provincial Center for Agricultural Microbial Resource Protection and Germplasm Innovation and Utilization, Nanjing Agricultural University, Nanjing 210095, China.
Science advances
|February 28, 2025
概括
基于草甘的除草剂会伤害土壤真菌,但不是通过向与植物相同的酶. 辅助剂Triton CG-110是主要的毒剂,破坏真菌膜并激活特定的途径.
科学领域:
- 环境微生物学环境微生物学
- 菌类学 菌类学是指菌类学.
- 生物化学 生物化学
背景情况:
- 基于草甘的除草剂 (GBHs) 广泛用于除草.
- 它们对土壤真菌群落的影响很大,尽管真菌有代谢草甘的能力.
- 在真菌中GBH毒性的精确机制仍然在很大程度上是未知的.
研究的目的:
- 调查基于草甘的除草剂对土壤真菌Trichoderma guizhouense影响的特定机制.
- 为了区分草甘与其辅助剂对真菌生理学的有毒作用.
- 阐明参与真菌对除草剂暴露的反应的信号通路.
主要方法:
- 对Roundup对*T. guizhouense*的影响的比较分析与工厂目标.
- 评估草甘和Triton CG-110对真菌生长和膜完整性的毒性.
- 关键酶和信号通路组件的基因表达分析 (例如,YPS1,TMK1,STE12).
主要成果:
- 在T. guizhouense中,Roundup的主要点不是5-enolpyruvyl-shikimate-3-phosphate合成酶,与植物不同.
- 除草剂辅助剂Triton CG-110对真菌细胞的毒性比草甘本身更高.
- 特里顿CG-110诱导吸收限制,激活蛋白酶YPS1,MAPK TMK1激素通路,以及转录因子STE12.
- STE12调节厄戈斯特醇生物合成,影响真菌膜的流动性和稳定性.
结论:
- 在 *T. guizhouense* 中,GBHs 的毒性主要归因于辅助剂 Triton CG-110,而不是草甘直接准石基酸盐通路.
- 这项研究揭示了真菌激素通路 (TMK1/STE12) 和厄戈斯特醇生物合成之间的新联系.
- 这种途径的激活对于在除草剂压力下保持血膜完整性至关重要.
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