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线粒体感知细菌乳酸并驱动中性粒体细胞外陷的释放

Ashley D Wise¹, Eden G TenBarge¹, Jessica D C Mendonça¹

¹Department of Microbiology, University of Tennessee, Knoxville, TN, USA.

Cell host & microbe

|February 28, 2025

概括

细菌乳酸信号中性粒细胞释放抗微生物陷. 中性粒细胞使用线粒体来检测这种信号,但这种过程在全身性红斑狼 (SLE) 患者中受损.

科学领域:

免疫学免疫学免疫学
微生物学微生物学
细胞生物学细胞生物学

背景情况:

中性粒细胞通过氧化压力创造了一个敌对的体环境.
金黄色葡萄球菌可以在中性粒细胞中持续存在,因此需要细菌感应机制.

研究的目的:

研究葡萄球菌乳酸盐作为代谢危险信号,触发中性粒细胞外细胞陷释放 (NETosis).
探索线粒体在感知细菌代谢活动中的作用.
为了检查系统性红斑狼 (SLE) 中性粒细胞中的NETosis缺陷.

主要方法:

研究了葡萄球菌乳酸在引发NETosis中的作用.
分析了线粒体协调与含有S. aureus的体.
检查了乳酸盐转化为pyruvate和随后的反应性氧物种生产.
从SLE患者的中性粒细胞中评估了NETosis.

主要成果:

葡萄球菌乳酸盐作为一种代谢危险信号,启动NETosis.
中性粒细胞线粒体感知细菌的乳酸盐,导致pyruvate转化和线粒体反应性氧物种的产生,这是NETosis的前体.
乳酸积累触发NETosis作为对各种细菌的反应.
SLE中性粒细胞表现出乳酸感应受损,导致对S. aureus有缺陷的NETosis,但异常的NETosis与亡残留物.

结论:

中性粒细胞利用线粒体作为感官器官来检测细菌的代谢活动.
这种传感机制决定了下游的抗菌过程,包括NETosis.
在SLE中性粒细胞中缺陷的乳酸感应有助于增加对细菌感染的易感性.

关键词:

网症 (NETosis) 是一种疾病.黄金葡萄球菌黄金葡萄球菌乳酸乳酸是一种乳酸.乳酸脱酶的使用方法线粒体中的线粒体.中性粒细胞外细胞陷中性粒细胞中性粒细胞.发酵体的发酵体是什么?反向电子运输的反向电子运输.系统性红血性狼 (Systemic Lupus Erythematosus) 是一种全身性狼.

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