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具有可控制和可调节分子重量的抗氧化的酶合成,使用磁性可回收的固定酸

Junhua Di¹, Yang Li¹, Yi Zhang¹

¹Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Hubei Key Laboratory of Lipid Chemistry and Nutrition, Hubei Hongshan Laboratory, Key Laboratory of Oilseeds Processing, Ministry of Agriculture, Wuhan 430062, China.

International journal of biological macromolecules

|February 28, 2025

概括

可磁性回收的固定 (Alcalase@SGO-PEGA) 提高了酶的稳定性和蛋白质水解的可重复使用性. 这种可持续的方法产生具有可调节分子重量的抗氧化,促进更清洁的蛋白质加工.

科学领域:

生物技术是生物技术.
酶工程是什么? 酶工程是什么?
蛋白质化学蛋白质化学

背景情况:

蛋白质的酶性水解产生生物活性,但酶的不稳定性和低可重复使用性阻碍了工业应用.
开发强大且可回收的酶系统对于可持续的蛋白质加工至关重要.

研究的目的:

开发一种磁性可回收的固定酶,用于高效的蛋白质水解.
提高的稳定性和可重复使用性,用于生产生物活性.
研究具有可调节分子重量的抗氧化的产生.

主要方法:

将酸盐固定在用聚乙烯糖醇二胺修改的超超磁性石墨烯氧化物上 (Alcalase@SGO-PEGA).
固定酶的稳定性 (热和pH耐受性) 和磁性恢复的表征.
使用Alcalase@SGO-PEGA.各种蛋白质来源的酶性水解.
评估生物活性的抗氧化特性 (ABTS基因清除和Fe2+化活性).

主要成果:

酸@SGO-PEGA表现出增强的热稳定性 (高达70°C) 和pH耐受性 (高达pH12).
使用磁场有效地恢复了固定化的酶,并在七个循环后保持了50%的活性.
从各种蛋白质来源成功地产生了具有不同分子量的生物活性.
获得的体显示出显著的抗氧化能力.

结论:

磁性可回收的固定 (Alcalase@SGO-PEGA) 提供了一个稳定且可重复使用的解决方案,用于酶性蛋白质水解.
这种方法使得可控分子重量的抗氧化的可持续生产成为可能.
这些发现促进了更清洁的蛋白质加工,并扩大了固定酶的应用.

关键词:

阿尔卡拉斯 (Alcalase) 是一种类植物.抗氧化剂类是一种抗氧化剂.石墨烯氧化物石墨烯氧化物固定化的酶.蛋白质的水解蛋白质的水解.

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