使用高通量测序 (HTS) 的元编码方法,在英国一年内评估人口暴露于空气中的真菌
Philippa Douglas1, Samuel Anees-Hill2, Sameirah Macchiarulo3
1Centre for Environmental Health and Sustainability, University of Leicester, Leicester, LE1 7LW, UK; Environmental Hazards and Emergencies Department, UK Health Security Agency, Harwell Campus, Chilton, Oxfordshire, OX11 0RQ, UK; Chief Scientist's Group, Environment Agency, Red Kite House, Wallingford, OX10 8BD, UK.
Environmental research
|February 28, 2025
概括
高通量测序 (HTS) 在英国识别了超过270种空中传播的真菌属,揭示了季节性和特定位置的差异. 这种方法提供了比传统显微镜更广泛的菌暴露视图,以获得公共卫生洞察力.
科学领域:
- 环境微生物学环境微生物学
- 菌类学 菌类学是指菌类学.
- 公共卫生 公共卫生
背景情况:
- 空气中的真菌与过敏和传染病有关.
- 显微镜是传统的,但在识别真菌类别方面有限.
- 高通量测序 (HTS) 提供了更广泛的环境分析.
研究的目的:
- 在英国两个地点使用HTS对12个月的空气传播真菌组成进行了表征.
- 为了比较城市和农村地区的真菌多样性和组成.
- 探索气象数据对空中真菌的影响.
主要方法:
- 在莱斯特 (城市) 和奇尔顿 (农村) 收集了一年多的空气样本.
- 使用具有内部转录间距区域 (ITS2) 和D1/D2 LSU元编码的HTS进行分析.
- 统计归算创建了一个12个月的数据集;气象数据纳入.
主要成果:
- HTS确定了272个真菌属,远远超过了显微镜.
- 菌的多样性和组成在不同地点之间是相似的,但有一些类型的差异.
- 空气温度和降水强烈影响了真菌的组成.
结论:
- HTS对于对空气中的真菌进行表征有价值,可以识别更广泛的种类.
- HTS可以作为查菌暴露和健康关联的工具.
- 建议在公共卫生干预中将HTS与定量方法结合起来.
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