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在巴西重新评估4型登革热病毒:基因组测序和分散动态,2011-2017年

Priscila Fonseca Ferreira¹, Luiz Fernando Almeida Machado², Jedson F Cardoso³

¹Programa de Pós Graduação em Biologia de Agentes Infecciosos e Parasitários, Instituto de Ciências Biológicas, Universidade Federal do Pará, CEP 66030-000 Belém, Pará, Brazil.

Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases

|February 28, 2025

概括

自2010年以来,登革热病毒4型 (DENV-4) 基因型4II已经在巴西蔓延,取代了4I.I.基因型. 基因组测序揭示了多次引入和病毒分散,突出了加强基因组监测以控制疫情的必要性.

科学领域:

病毒学病毒学
基因组学就是基因组学.
流行病学流行病学

背景情况:

第4型登革热病毒 (DENV-4) 在21世纪初在巴西重新出现,构成了严重的公共卫生威胁.
了解DENV-4的遗传多样性和时空传播对于有效的疾病控制至关重要.

研究的目的:

为了在2010-2017年间从巴西获得DENV-4分离物的遗传特征.
重新评估巴西DENV-4的时空动态和基因型分布.

主要方法:

在Ion Torrent平台上使用多重测序对24个人类DENV-4样本进行直接基因组测序.
遗传学和遗传地理分析以确定病毒血统,引入事件和分散模式.
对DENV-4基因型之间的基因突变进行比较分析.

主要成果:

所有新的DENV-4分离物只属于4II基因型,4II_B.1.1系,没有检测到之前识别的4I基因型.
植物地理分析表明,4II基因型在巴西的多次引入,在多个地区广泛分散.
观察到显著的遗传变异,包括18个基因组的独特非同义突变和7个基因组的改变氨基酸性质的突变.

结论:

这些发现表明,在巴西,DENV-4基因型4I可能会被4II基因型所取代.
DENV-4的传播是由复杂的病毒分散网络驱动的,强调了基因型分布的动态性.
先进的基因组测序对于监测DENV-4动态和为防治登革热爆发的公共卫生战略提供信息至关重要.

关键词:

在DENV-4中使用.基因组测序是指基因组的测序.基因组监测是指基因组的监测.血统 4II_B.1.11.1 血统 4II_B.1.1 血统 4II_B.1.1 血统

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