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两个色度测量LAMP系统用于基于核酸的诊断

Antao Sun¹, Petra Stejskalová², Xiaocheng Liu¹

¹Ministry of Education Key Laboratory of Micro and Nano Systems for Aerospace, School of Mechanical Engineering, Northwestern Polytechnical University, 127 West Youyi Road, 710072, Xi'an, Shaanxi, PR China.

Analytica chimica acta

|February 28, 2025

概括

这项研究介绍了一种新的8井色度循环介导同热放大 (LAMP) 系统,用于快速检测SARS-CoV-2. 该系统的精度与基于光的方法相当,提供了可访问的诊断工具.

科学领域:

分子生物学分子生物学
生物技术是生物技术.
医学诊断医学诊断医学诊断

背景情况:

开发用于传染病的先进诊断工具.
需要快速,可访问和简单的SARS-CoV-2检测方法.
基于光的传统测试的局限性.

研究的目的:

设计和评估用于检测SARS-CoV-2的自动化8井色度循环介导同热放大 (LAMP) 系统.
为了比较两种色度LAMP配置的性能与传统的光方法.
评估系统的速度,精度和简单性.

主要方法:

组装和测试使用不同的LED/传感器配置的两个不同的色度测量LAMP系统.
与基于光的循环介导同热放大 (LAMP) 在商用qPCR仪器上的比较.
使用不同SARS-CoV-2RNA度的验证.

主要成果:

颜值测量RT-LAMP测试实现了与光检测相比的临界值时间 (C_T) 值.
在一系列病毒载量 (大约) 中观察到一致和精确的C_T值. 1570 年至 157,000 个副本·μL-1).
该系统表现出了快速响应和精度,突出了其简单性.

结论:

开发的色度测量LAMP系统为SARS-CoV-2提供了一个快速,精确和可访问的诊断工具.
它对急诊诊断的潜力对于疫情应对非常重要.
该系统的适应性可以扩展到检测其他病原体.

关键词:

生物传感器和执行器测色仪检测检测的颜色测量检测循环介导的同热放大 (LAMP)护理点诊断的诊断方法快速测试技术快速测试技术在SARS-CoV-2的诊断上.

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