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在芽酵母中设计和表征艾伦因诱导表达系统

Junyi Wang¹, Jiaxue Ma¹, Xueyi Luo¹

¹State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Faculty of Medicine and Life Sciences, Xiamen University, Fujian, 361102, China.

Biotechnology for biofuels and bioproducts

|February 28, 2025

概括

研究人员在Saccharomyces cerevisiae中开发了一种新的阿兰因诱导基因表达系统. 这种艾伦因-GAL系统为酵母细胞工厂的生物化学品和蛋白质的生产提供了更好的控制和更高的产量.

科学领域:

微生物学微生物学
合成生物学合成生物学
生物技术是生物技术.

背景情况:

麦芽是生产生物化学物质和重组蛋白质的关键宿主.
酵母中的当前表达系统受到构成性或银河糖调节的促进体的限制.
有限的基因表达调节限制了酵母细胞工厂的生产率.

研究的目的:

在S. cerevisiae中设计和描述新型的艾伦因诱导表达系统.
通过结合可诱导艾伦和可诱导银河糖的系统来开发一种高度响应的遗传开关.
为了加强对微生物细胞工厂的基因表达控制和信号放大.

主要方法:

在S. cerevisiae中的一系列艾伦因诱导的DAL促进体的表征.
结合阿兰可诱导 (DAL) 和银河糖可诱导 (GAL) 系统,创建一个分层的遗传开关.
评估EGFP的表达和调节胡卜素的生产和细胞花.

主要成果:

开发了一种新的艾伦因-GAL系统,实现了 68.6.6 的 ON/OFF 比率.
与直接P_DAL2受控表达相比,艾伦-GAL系统显示出6.8倍的信号输出.
通过使用中心体等离子体观察到EGFP表达的增强的ON/OFF比率 (>67.2).
证明成功调节了胡卜素生产和细胞的发生.

结论:

在芽的酵母中,Allantoin诱导的DAL促进体被表征为芽酵母.
开发了一种分层的艾伦因-GAL系统,利用DAL2促进剂来调节银河糖调节.
艾伦因-GAL系统提供了比传统的P_DAL2受控表达更好的开/关比.
该系统具有低成本生产生物化学品和药品的潜力,使用阿兰因作为替代源.

关键词:

这种植物是Saccharomyces cerevisiae.艾伦·艾伦托恩在基因表达基因表达基因表达有层次的遗传电路.代谢工程是代谢工程.

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