综合的转录和代谢学揭示了Streptococcus thermophilus JM66在牛奶中的发酵过程中的变化:发酵特征,风味特征和代谢机制
Shasha Cheng1, Wenyan Li1, Hanying Yang1
1Key Laboratory of Dairy Science, Ministry of Education, College of Food Science and Engineering, Northeast Agricultural University, Harbin 150030, China.
Food research international (Ottawa, Ont.)
|March 1, 2025
概括
热菌 (Streptococcus thermophilus JM66) 产生66种挥发性代谢物,主要是类素,增强发酵牛奶的味道. 它的代谢途径,包括糖解和氨基酸代谢,是风味发展的关键.
科学领域:
- 食品微生物学 食品微生物学
- 代谢学 代谢学 代谢学
- 系统生物学 系统生物学
背景情况:
- 微生物的新陈代谢显著影响发酵牛奶的特性和味道.
- 热杆菌 (Streptococcus thermophilus) 是发酵牛奶生产中的一个关键的初始培养物.
研究的目的:
- 为了研究Streptococcus thermophilus JM66.6.的发酵特性,风味特征和代谢机制.
- 了解微生物代谢在牛奶发酵过程中形成风味化合物的作用.
主要方法:
- 发酵的多阶段动态监测.
- 多omics技术 (代谢学和转录学).
- 识别和量化挥发性代谢产物.
主要成果:
- 确定了66种挥发性代谢物,其中类如乙和nonanone是主要的风味化合物.
- 代谢分析显示了pyruvate,乳酸,氨基酸和脂肪酸代谢的变化.
- 转录组数据揭示了参与糖解,尿素循环和脂肪酸生物合成的基因的高表达.
结论:
- 热菌 (S. thermophilus JM66) 在发酵牛奶中显著促进了风味的发展.
- 代谢途径,特别是酸盐和氨基酸代谢,对于味道化合物形成至关重要.
- 提供了使用S. thermophilus JM66作为初始培养或改善菌株的基础.
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