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在从受感染的马中采集的血清样本中检测到马匹的alphaherpesvirus 1

Koji Tsujimura¹, Hiroshi Bannai¹, Yoshinori Kambayashi¹

¹Equine Research Institute, Japan Racing Association, Shimotsuke, Tochigi, Japan.

Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc

|March 1, 2025

概括

马类疹病毒1 (EqAHV1) 可以通过实时PCR (rtPCR) 在马类血清中检测到. 这项研究表明,血清是EqAHV1检测的可行的样本类型,尽管低病毒载荷可能会导致假阴性.

科学领域:

兽医病毒学兽医病毒学
马类传染病马类传染病
分子诊断学分子诊断

背景情况:

马类疹病毒1 (EqAHV1) 引起马的疾病,并通过病毒病传播.
EqAHV1通常与血液中的细胞相关,导致血清被忽视用于诊断测试.
像实时PCR (rtPCR) 这样的敏感方法尚未广泛应用于用于检测EqAHV1的马类血清.

研究的目的:

评估马类血清在使用rtPCR检测EqAHV1时的有用性.
为了在血清中检测EqAHV1时,将标准rtPCR与没有核酸净化的直接rtPCR进行比较.
评估基于血清的EqAHV1检测在实验感染和现场病例中的可靠性.

主要方法:

实时PCR (rtPCR) 用于从试验中感染EqAHV1的马的存档血清和外围血液单核细胞 (PBMC) 进行.
来自40匹发烧马的急性相场血清使用标准rtPCR和直接rtPCR进行了测试.
样本包括经过实验感染的马和11个ELISA阳性EqAHV1现场病例.

主要成果:

EqAHV1通过标准rtPCR检测到实验感染马的PBMC (3-6天) 和血清 (5-7天).
在11个ELISA阳性的野外血清中,有6个通过标准rtPCR呈阳性;其余的则呈阴性.
所有6个ELISA阳性,标准rtPCR阴性样本均在使用直接rtPCR而没有核酸净化时呈阳性.

结论:

马类血清是使用rtPCR检测EqAHV1的潜在样本类型.
没有核酸净化的直接rtPCR可以提高血清样本的检测灵敏度.
血清中病毒基因拷贝数量较低可能导致假阴性结果,需要仔细解释.

关键词:

马匹的阿尔法赫尔佩斯病毒1 在实时PCR中进行实时PCR.血液中的血清是血清.病毒病病毒病是什么病毒检测病毒检测

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