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相关实验视频

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高APEX1表达促进骨髓瘤细胞的增殖.

Yan Yu Lu1, Wen Lu1, Jie Zheng1

  • 1Division of Spinal Surgery, The First People's Hospital of Yulin (The Sixth Affiliated Hospital of Guangxi Medical University), No. 495 Mid-way of education, Yulin, 537000, Guangxi, China.

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阿普里尼克/阿普里米尼克外核酶1 (APEX1) 的高表达驱动骨髓瘤 (OS) 细胞的增殖,可能通过CD31. 针对APEX1可能为OS患者提供新的治疗策略.

关键词:
阿普里尼克/阿普里米尼克外核酶 1对DNA损伤的反应反应血小板内皮细胞粘附分子-1-1瘤的扩散 瘤的扩散骨质肉瘤是骨质肉瘤的一种疾病.

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科学领域:

  • 在瘤学瘤学.
  • 分子生物学分子生物学
  • 遗传学 是一个遗传学.

背景情况:

  • 骨肉瘤 (OS) 在几十年内显示出有限的生存率改善.
  • 分子向疗法为OS治疗提供了一个有希望的途径.
  • 在OS增殖中Apurinic/apyrimidinic外核酶1 (APEX1) 的作用尚未完全理解.

研究的目的:

  • 为了研究APEX1在OS中的表达.
  • 阐明APEX1影响OS细胞增殖和亡的机制.
  • 在OS中识别APEX1的潜在调节者.

主要方法:

  • 实时定量PCR (RT-qPCR) 用于基因表达分析.
  • 在MG-63细胞中进行基因淘汰的lentiviral载体 (APEX1-shRNA).
  • MTT,异种移植瘤生长和TUNEL测定用于扩散和亡的评估.
  • 生物信息学分析包括PPI网络和路径丰富.

主要成果:

  • 与正常组织和细胞相比,OS组织和细胞系中的APEX1表达显著更高.
  • 在体外和体内,APEX1敲击抑制了OS细胞的增殖,诱导了亡.
  • 阿佩克斯1突击降低了CD31的表达,但没有影响P53或Caspase3.
  • 生物信息学建议USF1或SP1作为APEX1转录的潜在调节者.

结论:

  • 升高的APEX1表达促进OS细胞的增殖,可能通过CD31.
  • APEX1的倒退抑制了OS的进展,并诱导了亡.
  • USF1或SP1可能调节APEX1转录及其在OS中DNA损伤反应途径中的作用.