七月3号细胞调节记忆和L-LTP依赖的内分泌网膜延伸到脊柱
Natsumi Ageta-Ishihara1, Yugo Fukazawa2, Fumiko Arima-Yoshida3
1Department of Biomolecular Science, Faculty of Science, Toho University, Funabashi, Chiba 274-8510, Japan; Department of Molecular Biology, Division of Biological Sciences, Nagoya University Graduate School of Science, Chikusa-ku, Nagoya 464-8602, Japan.
Cell reports
|March 1, 2025
概括
在SEPT3驱动下,平滑的内质网膜 (sER) 延伸到活跃的脊柱中,支着记忆巩固. 七蛋白亚单元SEPT3对于这个过程至关重要,它影响了突触可塑性和记忆持久性.
科学领域:
- 神经科学是一个神经科学.
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
背景情况:
- 记忆巩固涉及突触和电路水平的变化.
- 早期和晚期的长期强化 (E-LTP和L-LTP) 代表了突触巩固的阶段.
- 对于L-LTP持久性的机制,特别是在突触处,尚未完全理解.
研究的目的:
- 调查突触机制是后期期长期强化 (L-LTP) 持久性的基础.
- 为了确定参与L-LTP和记忆巩固的分子参与者,在穿孔路径-海马体牙状 (DG) 突触中.
主要方法:
- 在穿孔路径-DG突触处诱导L-LTP.
- 对细胞骨重塑的分析,包括actin和septin蛋白.
- 研究树突性脊柱中光滑内 плазма网膜 (sER) 的作用.
- 使用9月3日淘汰赛 (Sept3-/-) 鼠标来评估记忆巩固和突触功能.
- 使用电生理学和成像测量棘中的Ca2+反应.
主要成果:
- 在L-LTP诱导过程中,细胞骨通过actin和SEPT3.3进行重塑.
- 在L-LTP之后,SEPT3依赖的sER扩展到扩大的树突发生.
- 含有sER的棘对突触输入表现出增强的Ca2+反应.
- 9月3日淘汰赛损害了记忆巩固,并减少了含有sER的脊柱.
结论:
- 延伸到活跃的树突状棘中的光滑内质网膜 (sER) 是巩固记忆的关键突触机制.
- 隔膜子单元SEPT3在调解sER延伸和记忆持久性方面发挥着关键作用.
- 这项研究揭示了一个新的突触基础,用于将短暂的记忆转化为持久的记忆.
相关概念视频
Septins
1.8K
Septins are protein filaments forming the cytoskeleton along with the microtubules, microfilaments, intermediate filaments, and other accessory proteins. In 1971 while studying the cell division cycle in mutant Saccharomyces cerevisiae Harwell et al. first identified the septin-related genes playing a crucial role in yeast cytokinesis. Fluorescence microscopy revealed that these proteins localize at the budding neck as rings. These ring-like proteins were then named Septins by John Pringle, and...
1.8K
Role of Septins
1.7K
Septins are the recently discovered fourth major protein component of the cytoskeleton, along with microfilaments, microtubules, and intermediate filaments. These proteins can associate with other cytoskeletal filaments and carry out varied roles or can be free-floating in the cytoplasm.
Cellular Functions of Septins
Recent studies have revealed the multifaceted roles of septins in various cellular processes such as cytokinesis, ciliogenesis, and neurogenesis. Septins act as scaffolds and...
Cellular Functions of Septins
Recent studies have revealed the multifaceted roles of septins in various cellular processes such as cytokinesis, ciliogenesis, and neurogenesis. Septins act as scaffolds and...
1.7K
Tail-anchoring of Proteins in the ER Membrane
3.0K
Tail-anchored, or TA, proteins are estimated to make up to 3-5% of membrane proteins found in the eukaryotic cell. Such proteins have a single transmembrane domain located approximately 30 amino acid residues upstream from the C-terminal end. As a result, the signal recognition particle (SRP) cannot guide a TA protein to the ER membrane for cotranslational insertion. Hence, they are integrated into the ER membrane post-translationally using their C-terminal end as the anchor. TA proteins...
3.0K
Long-term Depression
2.5K
Long-term depression, or LTD, is one of the ways by which synaptic plasticity—changes in the strength of chemical synapses—can occur in the brain. LTD is the process of synaptic weakening that occurs over time between pre and postsynaptic neuronal connections. The synaptic weakening of LTD works in opposition to synaptic strengthening by long-term potentiation (LTP) and together are the main mechanisms that underlie learning and memory.
Calcium Ion Concentration Mechanism
If over...
Calcium Ion Concentration Mechanism
If over...
2.5K
Long-term Potentiation
2.7K
Long-term potentiation, or LTP, is one of the ways by which synaptic plasticity—changes in the strength of chemical synapses—can occur in the brain. LTP is the process of synaptic strengthening that occurs over time between pre and postsynaptic neuronal connections. The synaptic strengthening of LTP works in opposition to the synaptic weakening of long-term depression (LTD) and together are the main mechanisms that underlie learning and memory.
Hebbian LTP
LTP can occur when...
Hebbian LTP
LTP can occur when...
2.7K
ER Retrieval Pathway
3.7K
In the secretory pathway, vesicles transport proteins from one cellular compartment to another in forward transport to deliver the protein to its correct location. Occasionally, misfolded proteins and incorrect proteins escape their original compartments, and a retrieval pathway is used to return the escaped proteins to their original compartment.
The ER uses many checkpoints to prevent the entry of incorrectly folded or a resident protein as cargo onto a transport vesicle. These mechanisms...
The ER uses many checkpoints to prevent the entry of incorrectly folded or a resident protein as cargo onto a transport vesicle. These mechanisms...
3.7K


