在生物生产中,通过超热友好型古的进化和工艺工程来探索形式作为一种液态有机载体
Hae-Chang Jung1, Sung-Mok Lee1, Ji-In Yang2
1Marine Biotechnology & Bioresource Research Department, Korea Institute of Ocean Science and Technology, Busan 49111, Republic of Korea.
Bioresource technology
|March 1, 2025
概括
工程微生物有效地从酸盐中产生气,酸盐是液态有机的主要载体. 这一进步整合了二氧化碳电减,以实现可持续的经济.
科学领域:
- 生物技术是生物技术.
- 可再生能源可再生能源是可再生能源.
- 微生物工程 微生物工程
背景情况:
- (H2) 是一个有前途的可再生能源,但面临储存和交付的挑战.
- 液态有机载体 (LOHC),特别是酸盐,为存提供了可行的解决方案.
- 从酸盐生物生产是可持续能源的新兴领域.
研究的目的:
- 开发一种技术,利用超热友的archaeon *Thermococcus onnurineus* NA1.1.使用formate生产生物气.
- 通过微生物工程和发酵过程优化,提高气生产效率.
- 为了证明二氧化碳 (CO2) 电还原与生物生产的整合.
主要方法:
- 适应性进化 *Thermococcus onnurineus* NA1 创建一个工程菌株 (WTF-350 T) 的增强格式利用.
- 优化发酵条件,包括pH-stat,食批量模式和酸盐补充剂.
- 使用从CO2电还原中获得的酸作为生物生成的原料.
主要成果:
- 与野生类型相比,工程菌株WTF-350 T显示细胞生长,形式消耗和H2生产增强了3.6至4.0倍.
- 优化的发酵过程导致细胞密度和H2的生产率增加了2.0-2.5倍.
- 来自CO2电还原的酸被证明是生产生物的有效原料.
结论:
- 使用工程*Thermococcus onnurineus*从格中生产生物是可行的和高效的.
- 过程优化显著提高了的产量和生产率.
- 整合CO2电还原和生物生产为实现可持续经济提供了一个有希望的途径.
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