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Updated: May 24, 2025

A High-Throughput Luciferase Assay to Evaluate Proteolysis of the Single-Turnover Protease PCSK9
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基质识别和Lon蛋白酶的裂变部位偏好

Melanie Cragan1, Neha Puri1, A Wali Karzai1

  • 1Graduate Program in Molecular and Cellular Biology, Department of Biochemistry and Cell Biology, Center for Infectious Diseases, Stony Brook University, Stony Brook, New York, USA.

The Journal of biological chemistry
|March 1, 2025
PubMed
概括

研究人员为Lon蛋白酶确定了新的C端降解子,有助于发现细菌蛋白点. 这种Lon蛋白酶在基氨酸残留后优先分裂,这是跨物种保存的特征.

关键词:
与各种细胞活动 (AAA+) 相关的ATPases.单蛋白酶是一种蛋白质酶.蛋白质的平衡是蛋白质的平衡.蛋白质溶解过程中的蛋白质溶解.基质特异性 基质特异性

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