酸的消费激活了线粒体活动,甲基酸盐循环,并促进了人类病原体Histoplasma capsulatum细胞壁的变化
Luiz Paulo Araújo Santos1, Dayane Moraes1, Leandro do Prado Assunção1
1Laboratório de Biologia Molecular (LBM), Instituto de Ciências Biológicas (ICB), Universidade Federal de Goiás (UFG), Brazil.
Fungal biology
|March 1, 2025
概括
血清囊体通过甲基酸盐循环 (MCC) 代谢酸盐,激活特定的酶并改变细胞过程. 这种适应包括增加氧化应激和细胞壁重塑.
科学领域:
- 菌类学 菌类学是指菌类学.
- 医学真菌学 医学真菌学
- 在真菌病原体的产生过程中.
背景情况:
- 血清囊体会导致血清,一个显著的系统性菌根.
- 在感染期间,H. capsulatum利用营养物质产生烯-CoA,一种潜在的有毒分子.
- 甲基酸盐循环 (MCC) 对于真菌中的propionyl-CoA代谢至关重要.
研究的目的:
- 为了研究H. capsulatum对酸盐代谢的适应.
- 为了识别与酸利用有关的基因和蛋白质.
- 了解对酸盐的生理和细胞反应.
主要方法:
- 在MCC特定基因的分析中.
- 在酸盐培养细胞中对基因表达分析 (MCS,MCL).
- 对MCS的酶活性测定.
- 蛋白质组分析以确定受调节的蛋白质.
- 测量活性氧物种 (ROS) 和细胞壁变化.
主要成果:
- 在分析中发现了潜在的MCC酶基因 (MCS,MCD,MCL).
- 酸诱导了MCS和MCL基因表达,并增加了MCS酶活性.
- 蛋白质组学揭示了348种由酸调节的蛋白质,其中215种是上调调节的.
- 酸代谢导致ROS增加,细胞壁重塑,脂肪酸/氨基酸氧化发生变化.
结论:
- 在H. capsulatum中的propionate代谢激活了MCC通路.
- 这种真菌经历了显著的细胞适应,包括细胞壁的变化和氧化应激增加.
- 这些发现揭示了H. capsulatum在感染期间的代谢灵活性和对营养可用性的反应.
相关概念视频
00:53Products of the Citric Acid Cycle
98.1K
The cells of most organisms—including plants and animals—obtain usable energy through aerobic respiration, the oxygen-requiring version of cellular respiration. Aerobic respiration consists of four major stages: glycolysis, pyruvate oxidation, the citric acid cycle, and oxidative phosphorylation. The third major stage, the citric acid cycle, is also known as the Krebs cycle or tricarboxylic acid (TCA) cycle.
98.1K
02:36The Citric Acid Cycle
150.5K
The citric acid cycle, also known as the Krebs cycle or TCA cycle, consists of several energy-generating reactions that yield one ATP molecule, three NADH molecules, one FADH2 molecule, and two CO2 molecules.
150.5K
01:37The Citric Acid Cycle: Overview
15.8K
In aerobic organisms, the citric acid cycle is the second stage of cellular respiration wherein molecules derived from the breakdown of carbohydrates, proteins, and fats are oxidized into carbon dioxide and energy. This process is also known as the tricarboxylic acid (TCA) cycle as the first product of the cycle, citric acid, contains three carboxyl groups in its structure. Alternatively, this cycle is also referred to as the Krebs cycle, in honor of its discoverer Sir Hans Krebs.
The citric...
The citric...
15.8K
01:28The Citric Acid Cycle: Output
7.4K
The citric acid cycle is termed an amphibolic pathway as it operates both anabolically and catabolically. The cyclic reactions balance the flux of the substrates to provide an optimal concentration of NADH and ATP to the cell.
Regulation of Citric Acid Cycle
The citric acid cycle is regulated in several ways, including feedback inhibition, regulation of enzyme activities, and associated anaplerotic or cataplerotic pathways.
The primary substrate of the TCA cycle—acetyl CoA—is...
Regulation of Citric Acid Cycle
The citric acid cycle is regulated in several ways, including feedback inhibition, regulation of enzyme activities, and associated anaplerotic or cataplerotic pathways.
The primary substrate of the TCA cycle—acetyl CoA—is...
7.4K
01:20Fates of Pyruvate
8.3K
Pyruvate is the end product of glycolysis, where glucose is oxidized to pyruvate, simultaneously reducing NAD+ to NADH. Two molecules of ATP are also produced by substrate-level phosphorylation.
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
8.3K