由circGDSL诱导的OPR3表达调节了米 (Oryza sativa) 中的莉酸信号传递和铜耐受性
Jiajia Chen1, Mengwei Liu1, Yuqing Zhong2
1School of Chemistry and Life Science, Suzhou University of Science and Technology, Suzhou, 215011, China.
Plant physiology and biochemistry : PPB
|March 2, 2025
概括
这项研究揭示了大米中的特定循环RNA (circRNA) 如何通过激活素酸 (JA) 途径来增强对铜 (Cu) 的耐受性,从而减少有毒金属的积累.
科学领域:
- 植物分子生物学 植物分子生物学
- 环境应激反应环境应激反应
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 铜 (Cu) 是一种必需的微量营养素,但在高水平上有毒,影响植物生长和发育.
- 循环RNAs (circRNAs) 正在成为基因表达的关键调节者,包括应激反应.
- 了解重金属应力下的circRNA介导调节对于作物改善至关重要.
研究的目的:
- 为了研究circRNAs作为在铜 (Cu) 应力下在米中的竞争性内源RNAs (ceRNAs) 的作用.
- 为了确定涉及Cu耐受性的特定circRNA及其调节网络.
- 阐明一个关键的circRNA增强Cu应激抵抗的分子机制.
主要方法:
- 水培实验用于评估生理参数和Cu积累.
- RNA测序 (RNA-seq) 用于识别差异表达的环RNAs.
- 一个circRNA介导的ceRNA网络的构建和拓分析.
- 使用RT-qPCR,基因过度表达和生理分析进行实验验证.
主要成果:
- 通过RNA-seq鉴定出1051个环RNA,其中26个在Cu应力下差异表达.
- 构建了一个Cu-响应的ceRNA网络,涉及16个circRNA,34个miRNA和126个mRNA.
- 确定circGDSL/miR1850.1/OPR3通路作为一个关键的监管枢纽.
- 过度表达circGDSL通过激活素酸 (JA) 合成,增加Cu耐受性,减少Cu吸收.
结论:
- 一种新的Cu-响应性circRNA,circGDSL,充当miRNA海绵,激活大米中的JA通路.
- 这种circRNA介导的JA激活通过限制金属积累来增强大米对Cu毒性的耐受性.
- 这些发现提供了对基于circRNA的调控机制的见解,以改善作物应激弹性.
相关概念视频
Cell Signaling in Plants
5.6K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
5.6K
Responses to Salt Stress
12.9K
Salt stress—which can be triggered by high salt concentrations in a plant’s environment—can significantly affect plant growth and crop production by influencing photosynthesis and the absorption of water and nutrients.
12.9K
Transgenic Plants
7.1K
Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
7.1K
Regulation of Transpiration by Stomata
27.7K
During photosynthesis, plants acquire the necessary carbon dioxide and release the produced oxygen back into the atmosphere. Openings in the epidermis of plant leaves is the site of this exchange of gasses. A single opening is called a stoma—derived from the Greek word for “mouth.” Stomata open and close in response to a variety of environmental cues.
27.7K
Adaptations that Reduce Water Loss
25.1K
Though evaporation from plant leaves drives transpiration, it also results in loss of water. Because water is critical for photosynthetic reactions and other cellular processes, evolutionary pressures on plants in different environments have driven the acquisition of adaptations that reduce water loss.
25.1K
IP3/DAG Signaling Pathway
11.8K
Membrane lipids such as phosphatidylinositol (PI) are precursors for several membrane-bound and soluble second messengers. Specific kinases phosphorylate PI and produce phosphorylated inositol phospholipids. One such inositol phospholipids are the phosphatidylinositol-4,5 bisphosphate [PI(4,5)P2], present in the inner half of the lipid bilayer. Upon ligand binding, GPCR stimulates Gq proteins to turn on phospholipase Cꞵ. Activated phospholipase Cꞵ cleaves PI(4,5)P2 and...
11.8K


