相关实验视频

Updated: May 24, 2025

A Primary Human Trophoblast Model to Study the Effect of Inflammation Associated with Maternal Obesity on Regulation of Autophagy in the Placenta

A Primary Human Trophoblast Model to Study the Effect of Inflammation Associated with Maternal Obesity on Regulation of Autophagy in the Placenta

Published on: September 27, 2017

暴露于1 - 尼托烯对热囊细胞生物行为的影响

Chuting Wang¹, Long Zhang¹, Daidi Gui¹

¹Department of Occupational Health and Environmental Health, School of Public Health, Anhui Medical University, 81 Meishan Road, Hefei, Anhui 230032, China.

Reproductive toxicology (Elmsford, N.Y.)

|March 2, 2025

概括

作为PM2.5的组成部分的1-基烯 (1-NP) 损害了热囊细胞的功能,如入侵和迁移. 这项研究揭示了1-NP通过改变表皮细胞-介质细胞过渡 (EMT) 和Wnt/β-catenin通路来影响这些行为.

科学领域:

环境健康环境健康
毒理学毒理学毒理学
生殖生物学生殖生物学

背景情况:

颗粒物2.5 (PM2.5) 含有有毒的化合物,如1-尼托烯 (1-NP).
1-NP对人类健康,特别是孕妇的健康产生不利影响,但其对 trofhoblast 细胞的机制尚未完全理解.

研究的目的:

为了研究1 - 尼托烯 (1-NP) 影响热囊细胞行为机制.
探索1-NP对细胞克隆性,入侵和迁移的影响.
分析1-NP.的表皮质-介质细胞过渡 (EMT) 和Wnt/β-catenin通路的调节.

主要方法:

HTR8/Svneo热细胞被暴露在不同度的1-NP (0,5,10,20μM).
评估了包括克隆性,侵入性和迁移在内的细胞功能.
西部斑点分析用于评估EMT和Wnt/β-catenin信号通路中的关键蛋白质的表达水平.

主要成果:

1-NP显著抑制了HTR8/Svneo细胞的克隆性,侵入性和迁移,以剂量依赖的方式.
暴露于1-NP导致E-cadherin增加和Vimentin表达减少,表明EMT调节.
1-NP改变了Wnt/β-catenin路径组件,具有升高的β-catenin和降低的APC表达.

结论:

1-尼托烯对热囊细胞的克隆性,入侵和迁移产生负面影响.
这些效应通过表皮细胞-介质细胞过渡 (EMT) 和Wnt/β-catenin信号通路的调节来调节.
这些发现为1-NP对热囊细胞的生物学影响以及怀孕期间的潜在风险提供了新的见解.

关键词:

一-尼特罗皮尔烯 (Nitropyrene) 是一种1 - 尼特罗皮尔烯.在EMT中,EMT是EMT.人体胆管 trofhoblast 细胞的人体胆管 trofhoblast 细胞在Wnt/β-catenin中使用.

更多相关视频

In Vitro Assays to Evaluate the Migration, Invasion, and Proliferation of Immortalized Human First-trimester Trophoblast Cell Lines

In Vitro Assays to Evaluate the Migration, Invasion, and Proliferation of Immortalized Human First-trimester Trophoblast Cell Lines

Published on: March 5, 2019

Human Primary Trophoblast Cell Culture Model to Study the Protective Effects of Melatonin Against Hypoxia/reoxygenation-induced Disruption

Human Primary Trophoblast Cell Culture Model to Study the Protective Effects of Melatonin Against Hypoxia/reoxygenation-induced Disruption

Published on: July 30, 2016

相关概念视频

Teratogenicity

Teratogenicity

The ability of a drug to produce structural deformations and functional abnormalities in the developing embryo or the fetus is called teratogenicity, and the drug producing this effect is known as a teratogen. Teratogenic effects include stillbirth, miscarriage, intrauterine growth restriction, and neurocognitive delay. A teratogen may affect the embryo at different stages of development, which is important in determining the type and extent of the damage. During blastocyst formation, the early...