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在小鼠前脑的发育和成熟前脑中,GPER/GPR30记者表达的空间和时间模式

Meimei Wu¹, Wenxin He², Huashan Gong¹

¹Department of Gastroenterology, Songjiang Research Institute, Songjiang Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 201600, China; Department of Anatomy and Physiology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.

Brain research bulletin

|March 2, 2025

概括

G蛋白结合雌激素受体 (GPER) 在成年和发育前脑中广泛存在,特别是在嗅觉和突出网络的神经元中. 它的表达表明GPER在大脑发育和功能中起着关键作用.

科学领域:

神经科学是一个神经科学.
内分泌学在内分泌学.
发展生物学发展生物学

背景情况:

雌激素对神经发育和功能至关重要.
G蛋白结合雌激素受体 (GPER/GPR30) 是大脑中的主要雌激素受体.
在发育和成熟的大脑中,GPER的分布和功能仍然不完全理解.

研究的目的:

描述GPER在发育和成熟的小鼠前脑中的表达.
识别表达GPER的特定细胞类型和大脑区域.
研究神经发育早期GPER表达的时间模式.

主要方法:

使用了Gper基因记者小鼠 (Gper-cre与Ai14(RCL-tdT) -D或R26-ZsGreen交叉).
采用光在位杂交 (FISH/RNAscope) 和免疫组织化学 (IHC).
进行了神经追踪研究.

主要成果:

在前脑神经元,原质星体细胞,壁细胞和表皮细胞中检测到GPER记者.
表达GPER的神经元集中在嗅觉系统和突出网络中.
早期发育 (P1-P10) 期间的GPER表达与神经发生,增殖和迁移相关.

结论:

在成年和发育前脑中,GPER在多种细胞类型中表达.
空间和时间表达模式表明GPER调节了嗅觉系统,突出网络和大脑血管系统的发展和功能.
表达GPER的神经元主要是远程激发性金字塔神经元.

关键词:

雌激素是一种雌激素.与G蛋白结合的雌激素受体受体神经发育的神经发育嗅觉系统是一种嗅觉系统.Salience 网络是一个突出网络.

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相关概念视频

Reporter Genes

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.